Document Detail


Effects of multiple courses of antenatal corticosteroids on regional brain and somatic tissue water content in ovine fetuses.
MedLine Citation:
PMID:  15051036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To study the effects of single and multiple courses of antenatal corticosteroids on tissue water content in ovine fetuses. METHODS: After chronic catheterization of the ewes and fetuses, the ewes were randomly assigned to receive single or multiple courses of dexamethasone or placebo beginning at 104-106 or 76-78 days' gestation, respectively. In the single course groups, the ewes received dexamethasone (6 mg, n = 6) or placebo (n = 6) as four intramuscular injections every 12 hours over 48 hours. The fetal tissues were harvested for water content determination 66 hours after the first injection of dexamethasone or placebo was given. In the multiple-course groups, the ewes received the same treatment (dexamethasone, n = 10, or placebo, n = 8), once a week for 5 weeks starting at 76-78 days' gestation. In these groups, the tissues were harvested 66 hours after the first the injection of the fifth and last treatment course. In both groups, tissues were harvested at 106-107 days' gestation. Tissue water content was determined by wet-to-dry weight ratio in brain (cerebral cortex, caudate nucleus, cerebellum, midbrain, and medulla) and somatic tissues (kidney, liver, muscle, and skin). RESULTS: Water content in the brain regions (cerebellum and medulla) was lower (P <.05) in fetuses of dexamethasone-treated ewes than placebo-treated ewes after the multiple course but not the single course. Water content of somatic tissue was lower (P <.05) in fetuses of dexamethasone-treated ewes than placebo-treated ewes after the multiple courses, and in the liver after a single course. CONCLUSION: Dexamethasone treatment of ewes at 70% of gestation results in decreased regional brain water content in the fetuses after multiple but not single treatment courses, in somatic tissues (kidney, liver, muscle, and skin) after multiple courses, and in the liver after a single course.
Authors:
Barbara S Stonestreet; Shannon Watkins; Katherine H Petersson; Grazyna B Sadowska
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the Society for Gynecologic Investigation     Volume:  11     ISSN:  1071-5576     ISO Abbreviation:  J. Soc. Gynecol. Investig.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-30     Completed Date:  2004-11-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9433806     Medline TA:  J Soc Gynecol Investig     Country:  United States    
Other Details:
Languages:  eng     Pagination:  166-74     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Brown University Medical School, Women and Infants' Hospital of Rhode Island, Providence 02905-2499, USA. BStonest@wihri.org
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MeSH Terms
Descriptor/Qualifier:
Adrenal Cortex Hormones / administration & dosage*
Animals
Blood Glucose / analysis
Blood Pressure
Body Water / drug effects*
Brain / anatomy & histology,  embryology*
Carbon Dioxide / analysis
Dexamethasone / administration & dosage
Female
Fetal Blood / chemistry
Fetus / drug effects*
Gestational Age
Heart Rate, Fetal
Hematocrit
Hydrocortisone / blood
Hydrogen-Ion Concentration
Insulin / blood
Osmolar Concentration
Oxygen / analysis
Placebos
Potassium / blood
Pregnancy
Sheep
Sodium / blood
Grant Support
ID/Acronym/Agency:
R01-HD34618/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Adrenal Cortex Hormones; 0/Blood Glucose; 0/Placebos; 11061-68-0/Insulin; 124-38-9/Carbon Dioxide; 50-02-2/Dexamethasone; 50-23-7/Hydrocortisone; 7440-09-7/Potassium; 7440-23-5/Sodium; 7782-44-7/Oxygen

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