Document Detail

Effects of modulation of nitric oxide on rat diaphragm isotonic contractility during hypoxia.
MedLine Citation:
PMID:  12391124     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO) is essential for optimal myofilament function of the rat diaphragm in vitro during active shortening. Little is known about the role of NO in muscle contraction under hypoxic conditions. Hypoxia might increase the NO synthase (NOS) activity within the rat diaphragm. We hypothesized that NO plays a protective role in isotonic contractile and fatigue properties during hypoxia in vitro. The effects of the NOS inhibitor N(G)-monomethyl-l-arginine (l-NMMA), the NO scavenger hemoglobin, and the NO donor spermine NONOate on shortening velocity, power generation, and isotonic fatigability during hypoxia were evaluated (Po(2) approximately 7 kPa). l-NMMA and hemoglobin slowed the shortening velocity, depressed power generation, and increased isotonic fatigability during hypoxia. The effects of l-NMMA were prevented by coadministration with the NOS substrate l-arginine. Spermine NONOate did not alter isotonic contractile and fatigue properties during hypoxia. These results indicate that endogenous NO is needed for optimal muscle contraction of the rat diaphragm in vitro during hypoxia.
Xiaoping Zhu; Leo M A Heunks; Herwin A Machiels; Leo Ennen; P N Richard Dekhuijzen
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2002-10-18
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  94     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-01-17     Completed Date:  2003-07-21     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  612-20     Citation Subset:  IM    
Department of Pulmonary Diseases, University Medical Centre Nijmegen, The Netherlands.
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MeSH Terms
Anoxia / physiopathology*
Diaphragm / physiopathology*
Enzyme Inhibitors / pharmacology
Hemoglobins / pharmacology
Isotonic Contraction* / drug effects
Muscle Fatigue / physiology*
Nitric Oxide / antagonists & inhibitors,  metabolism*
Nitric Oxide Donors / pharmacology
Nitrogen Oxides
Physical Endurance
Rats, Wistar
Spermine / analogs & derivatives*,  pharmacology
Time Factors
omega-N-Methylarginine / pharmacology
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Hemoglobins; 0/Nitric Oxide Donors; 0/Nitrogen Oxides; 10102-43-9/Nitric Oxide; 136587-13-8/spermine nitric oxide complex; 17035-90-4/omega-N-Methylarginine; 71-44-3/Spermine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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