Document Detail

Effects of mitomycin C on macromolecular synthesis in Escherichia coli.
MedLine Citation:
PMID:  5335968     Owner:  NLM     Status:  MEDLINE    
When cells of Escherichia coli B growing in a glucose-synthetic medium were treated with mitomycin C, the effects produced by the antibiotic varied, depending on the concentration. When the concentration was reduced to less than 0.1 mug/ml, the action of the antibiotic was bacteriostatic; cell elongation resulted, but no effect on the synthesis of cellular macromolecules was apparent. At higher levels (more than 5 mug/ml), mitomycin C was highly bactericidal and inhibited deoxyribonucleic acid synthesis almost completely. The exposure of growing cells to a bactericidal level of mitomycin C resulted also in a delayed inhibition of the synthesis of ribonucleic acid (RNA) and protein. The capacity of the treated cells to synthesize beta-galactosidase inducibly in a medium free from a carbon source remained constant for the first 30 min and then was destroyed progressively with time. Prolonged incubation with the bactericidal level of mitomycin C caused a degradation of cellular nucleic acids, particularly RNA. The degraded nucleic acid components were eventually released into the medium.
H Suzuki; W W Kilgore
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  93     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1967 Feb 
Date Detail:
Created Date:  1967-05-12     Completed Date:  1967-05-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  675-82     Citation Subset:  IM    
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MeSH Terms
Bacterial Proteins / biosynthesis
Carbon Isotopes
DNA, Bacterial / biosynthesis*
Escherichia coli / drug effects*,  growth & development,  metabolism*
Glycoside Hydrolases / analysis
Mitomycins / pharmacology*
RNA, Bacterial / biosynthesis*
Reg. No./Substance:
0/Bacterial Proteins; 0/Carbon Isotopes; 0/DNA, Bacterial; 0/Mitomycins; 0/RNA, Bacterial; EC 3.2.1.-/Glycoside Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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