Document Detail

Effects of mineral trioxide aggregate on cell survival, gene expression associated with mineralized tissues, and biomineralization of cementoblasts.
MedLine Citation:
PMID:  19345796     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to investigate the effects of mineral trioxide aggregate (MTA) on survival, mineralization, and expression of mineralization-related genes of cementoblasts. Immortalized cementoblasts (OCCM) were maintained with Dulbecco modified Eagle medium containing 10% fetal bovine serum. Methyl-thiazol-diphenyl-tetrazolium experiments were performed at 24 and 72 hours to evaluate bioactive components released by MTA (0.002-20 mg/mL) on the cell survival of OCCM. Von Kossa staining was used to evaluate biomineralization of OCCM cells. Images of cementoblasts were taken on day 3 by using inverted microscopy. Gene transcripts for bone sialoprotein (BSP), OCN, collagen type I (COL I), and osteopontin (OPN) were evaluated on days 3 and 5 by using semiquantitative reverse transcriptase polymerase chain reaction. The 20 mg/mL concentration of MTA was toxic for OCCM cells, whereas other concentrations of MTA tested exhibited similar cell numbers when compared with control group, and the 0.02 mg/mL concentration of MTA increased OCCM cell survival at 72 hours. Although an apparent decrease in mineralization was observed in the highest 3 concentrations of MTA used, 0.02 and 0.002 mg/mL concentrations of MTA induced greater biomineralization of OCCM cells than seen in the control. Moreover, increased BSP and COL I mRNA expression was observed at 0.02 and 0.002 mg/mL concentrations of MTA. MTA did not have a negative effect on the viability and morphology of cementoblasts and induced biomineralization of cementoblasts at the concentrations of 0.02 and 0.002 mg/mL. Based on these results MTA can be considered as a favorable material regarding cell-material interaction.
Sema S Hakki; S Buket Bozkurt; Erdogan E Hakki; Sema Belli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endodontics     Volume:  35     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-07-07     Revised Date:  2010-02-04    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  513-9     Citation Subset:  D    
Faculty of Dentistry, Department of Periodontology, Selcuk University, Konya, Turkey.
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MeSH Terms
Aluminum Compounds / pharmacology*
Calcification, Physiologic / drug effects*,  genetics
Calcium Compounds / pharmacology*
Cell Line, Transformed
Cell Survival / drug effects
Cementogenesis / drug effects*
Collagen Type I / biosynthesis,  genetics
Dental Cementum / drug effects*
Dose-Response Relationship, Drug
Drug Combinations
Gene Expression / drug effects
Osteocalcin / biosynthesis,  genetics
Osteopontin / biosynthesis,  genetics
Oxides / pharmacology*
Reverse Transcriptase Polymerase Chain Reaction
Root Canal Filling Materials / pharmacology*
Sialoglycoproteins / biosynthesis,  genetics
Silicates / pharmacology*
Reg. No./Substance:
0/Aluminum Compounds; 0/Calcium Compounds; 0/Collagen Type I; 0/Drug Combinations; 0/Oxides; 0/Root Canal Filling Materials; 0/Sialoglycoproteins; 0/Silicates; 0/integrin-binding sialoprotein; 0/mineral trioxide aggregate; 104982-03-8/Osteocalcin; 106441-73-0/Osteopontin

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