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Effects of mevinolin on cell cycle progression and viability of tobacco BY-2 cells.
MedLine Citation:
PMID:  15494054     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Mevinolin, an inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase, was used to study the importance of mevalonic acid (MVA) for cell cycle progression of tobacco (Nicotiana tabacum L.) BY-2 cells. After treatment with 5 microM mevinolin, the cell cycle progression was completely blocked and two cell populations accumulated (80% in phase G0/G1 and 20% in G2/M). The arrest could be released by subsequent addition of MVA. Effects were compared to those caused by aphidicolin, an inhibitor of alpha-like DNA polymerases that blocks cell cycle at the entry of the S phase. The 80% proportion of mevinolin-treated TBY-2 cells was clearly arrested before the aphidicolin-inducible block. By the aid of a double-blocking technique, it was shown that the mevinolin-induced cell arrest of highly synchronized cells was due to interaction with a control point located at the mitotic telophase/entry G1 phase. Depending on the developmental stage, mevinolin induced rapid cell death in a considerable percentage of cells. Mevinolin treatment led to a partial synchronization, as shown by the increase in mitotic index. The following decrease was correlated with the above-mentioned induction of cell death.
Authors:
A Hemmerlin; T J Bach
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  14     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  2004-10-20     Completed Date:  2005-05-03     Revised Date:  2006-11-07    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  65-74     Citation Subset:  -    
Affiliation:
Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, Département d'Enzymologie Cellulaire et Moléculaire, Institut de Botanique, Université Louis Pasteur, 28 rue Goethe, F67083 Strasbourg, France.
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