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Effects of methylphenidate: the cellular point of view.
MedLine Citation:
PMID:  21432609     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The psychostimulant methylphenidate (MPH) is the first choice of treatment in attention-deficit hyperactivity disorder and is based mainly on inhibition of dopamine transporter (DAT). Nonetheless, the complete cellular effects of MPH are still unknown. We attempted to determine whether MPH influences neurotransmitter levels, synaptic gene expression, and cell proliferation in a dose-dependent manner in rat pheochromocytoma cells (PC12) lacking DAT. PC12 were treated in a dose-dependent manner with MPH. Gene expression level of synaptotagmin (Syt) 1 and 4, syntaxin 1a (Stx1a), and synaptic vesicle glycoprotein 2C (SV2C) was measured using quantitative real-time RT-PCR. Different Neurotransmitter release was measured using high-performance liquid chromatography (HPLC). Differences in cell proliferation were evaluated via BrdU incorporation. Treatment with low-dose MPH (1-100 nM) altered intra-/extracellular neurotransmitter levels, down-regulated all investigated genes as well as enhanced cell proliferation significantly. These data point to diverse effects of MPH on cell metabolism independent of inhibiting DAT.
Authors:
Jasmin Bartl; Pille Link; Corinna Schlosser; Manfred Gerlach; Angelika Schmitt; Susanne Walitza; Peter Riederer; Edna Grünblatt
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Publication Detail:
Type:  Journal Article     Date:  2010-11-10
Journal Detail:
Title:  Attention deficit and hyperactivity disorders     Volume:  2     ISSN:  1866-6647     ISO Abbreviation:  Atten Defic Hyperact Disord     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-03-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101491944     Medline TA:  Atten Defic Hyperact Disord     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  225-32     Citation Subset:  IM    
Affiliation:
Clinical Neurochemistry Laboratory, Clinic and Policlinic for Psychiatry, Psychosomatic and Psychotherapy, University of Würzburg, Füchsleinstr. 15, 97080, Würzburg, Germany.
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