Document Detail


Effects of methoxyverapamil on the stimulation by Ca2+, Sr2+ and Ba2+ and on the inhibition by Mg2+ of catecholamine release from the adrenal medulla.
MedLine Citation:
PMID:  465897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1 Bovine adrenal glands were perfused with Ca2+-free Locke solution and catecholamine release was induced either by the introduction of Ca2+, Sr2+ or Ba2+ into the perfusion fluid or by the substitution of Na+ by an osmotically equivalent amount of sucrose. 2 Methoxyverapamil (D600) at a concentration of 3 X 10(-4) M blocked the release of catecholamines in response to Ca2+, Sr2+ or Ba2+ stimulation but failed to block the release evoked by the omission of Na+. 3 Mg2+ (10 to 20 mM) blocked the release induced by Na+-deprivation; however, this inhibitory effect of Mg2+ was not modified by D600. 4 D600 blocked the increase in the efflux of 45Ca from the perfused gland induced by the introduction of Ca2+ into the perfusion fluid and blocked the uptake of 45Ca into adrenal medullary slices induced by K+ depolarization. 5 The results suggest that Ca2+, Sr2+ and Ba2+ may enter the chromaffin cell through the same channel and that this channel is blocked by D600. Mg2+ may enter the cell through the same Ca2+ channel but with a high rate of permeation or it may enter through a channel which is resistant to D600. Alternatively, Mg2+ may exert this inhibitory effect at an extracellular site.
Authors:
J Aguirre; J Falutz; J E Pinto; J M Trifaró
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  British journal of pharmacology     Volume:  66     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1979 Aug 
Date Detail:
Created Date:  1979-10-26     Completed Date:  1979-10-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  591-600     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adrenal Medulla / drug effects*,  secretion
Animals
Barium / pharmacology
Calcium / pharmacology*
Catecholamines / secretion*
Cations, Divalent / pharmacology*
Cattle
Drug Interactions
Gallopamil / pharmacology*
Magnesium / pharmacology
Perfusion
Strontium / pharmacology
Verapamil / analogs & derivatives*
Chemical
Reg. No./Substance:
0/Catecholamines; 0/Cations, Divalent; 16662-47-8/Gallopamil; 52-53-9/Verapamil; 7439-95-4/Magnesium; 7440-24-6/Strontium; 7440-39-3/Barium; 7440-70-2/Calcium
Comments/Corrections

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