| Effects of membrane ribonuclease and 3'-nucleotidase on the digestion of polyuridylic acid by rat liver plasma membrane. | |
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MedLine Citation:
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PMID: 89 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Fragments of isolated rat liver plasma membrane possess a ribonuclease activity which at pH 7.8 in the presence of 10 mM EDTA can digest polyuridylic acid (poly(U)) and polycytidylic acid (poly(C)) but not polyadenylic acid (poly(A)) and polyguanylic acid (poly(G)). Under these conditions, the membrane preparation does not degrade native or denatured DNA. 2. The products of the reaction with poly(U) (10 mM EDTA present) can be separated on DEAE-Sephadex into oligonucleotides of increasing chain length. Most of the products are di- to hexa-nucleotides which contain terminal 3'-phosphate groups. 3. When EDTA is not present (pH 7.8 or 8.8) the plasma membrane preparation degrades both poly(A) and poly(U). With poly(A) the product is all nucleoside while with poly(U) as substrate most of the product is nucleoside, but also some oligonucleotides are produced. 4. The ribonuclease releases acid soluble products very slowly from high concentrations of poly(U) (mg/ml). 5. Uridine trinucleotide with and without a terminal 3'-phosphate group is degraded by rat liver plasma membrane. The trinucleotide diphosphate is rapidly hydrolyzed to nucleoside while the trinucleotide itself is slowly digested and yields intermediate products, including nucleoside. |
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Authors:
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N N Aronson; A Yannarell |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 413 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1975 Nov |
Date Detail:
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Created Date: 1976-02-20 Completed Date: 1976-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 135-42 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / enzymology* DNA / metabolism Edetic Acid / pharmacology Hydrogen-Ion Concentration Nucleotidases / metabolism* Oligoribonucleotides / analysis Phosphates Poly A / metabolism Poly C / metabolism Poly G / metabolism Poly U / metabolism* Rats Ribonucleases / metabolism* Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Oligoribonucleotides; 0/Phosphates; 24937-83-5/Poly A; 25191-14-4/Poly G; 27416-86-0/Poly U; 30811-80-4/Poly C; 60-00-4/Edetic Acid; 9007-49-2/DNA; EC 3.1.-/Ribonucleases; EC 3.1.3.-/Nucleotidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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