Document Detail

Effects of megakaryocyte growth and development factor (thrombopoietin) on liver endothelial cells in vitro.
MedLine Citation:
PMID:  10458926     Owner:  NLM     Status:  MEDLINE    
Thrombopoietin (TPO) is the major regulator of growth and differentiation of megakaryocytes. Recent studies have shown that TPO also has activity on hematopoietic lineages other than megakaryocytes. However, little is known about the effects of TPO on nonhematopoietic cells expressing the TPO receptor, such as endothelial cells (EC). We have previously shown that specific murine liver EC (LEC-1) located in the hepatic sinusoids coexpress TPO and its receptor, c-mpl. Likewise, we showed that TPO has a proliferative effect on LEC-1. In this study, we have further examined the effects of TPO on other biological functions of LEC-1. Stimulation with TPO induced secretion of proinflammatory cytokines (i.e., IL-1beta, IL-6, TNF-alpha) from LEC-1. TPO-induced proliferation of LEC-1 was synergistically enhanced with the addition of TNF-alpha. TPO also induced the proliferation of LEC-1 in the presence of IFN-gamma, which alone inhibited the growth of these cells. TPO has no effect on other endothelial cell functions such as nitric oxide production and adhesion molecule expression. These observations establish a novel activity of TPO on murine liver endothelial cells in terms of inducing cytokine production by these cells. Our results suggest that this cytokine may act synergistically with other cytokines to induce LEC-1 proliferation.
J E Cardier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microvascular research     Volume:  58     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-18     Completed Date:  1999-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  108-13     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, 1020-A, Venezuela.
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MeSH Terms
Cell Division / drug effects
Cell Line
Cytokines / biosynthesis
Endothelium, Vascular / cytology,  drug effects,  immunology
Inflammation Mediators / metabolism
Interferon-gamma, Recombinant / pharmacology
Interleukin-1 / biosynthesis
Interleukin-6 / biosynthesis
Liver / blood supply*,  cytology,  drug effects*
Recombinant Proteins / pharmacology
Thrombopoietin / pharmacology*
Tumor Necrosis Factor-alpha / biosynthesis,  pharmacology
Reg. No./Substance:
0/Cytokines; 0/Inflammation Mediators; 0/Interferon-gamma, Recombinant; 0/Interleukin-1; 0/Interleukin-6; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 9014-42-0/Thrombopoietin

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