Document Detail

Effects of medium glutamine, glutamate, and ammonia on glutamine synthetase activity in cultured mouse astroglial cells.
MedLine Citation:
PMID:  2894616     Owner:  NLM     Status:  MEDLINE    
Mouse astroglial cells were grown during the last week of culture in either glutamine-free or glutamine-containing medium. The addition of cortisol to the glutamine-containing medium resulted in a doubling of astroglial glutamine synthetase (GS) activity. Withdrawal of glutamine from the medium resulted in a 50% elevation of GS and addition of cortisol to such a medium resulted in a further increase in GS which was not additive to glutamine withdrawal. Both in glutamine-free and glutamine-containing medium, the addition of glutamate resulted in a depression of both basal and cortisol induced GS activity. The simultaneous addition of ammonia plus glutamate to the culture medium ameliorated the glutamate mediated depressive effects on cortisol induced but not basal GS activity. Glutamine withdrawal from the culture medium resulted in an astroglial protein deficit. The addition of ammonia to the medium considerably reduced this deficit and the addition of glutamate completely eliminated this protein deficit.
B H Juurlink
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  12     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-04-13     Completed Date:  1988-04-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1053-8     Citation Subset:  IM    
Department of Anatomy, University of Saskatchewan, Saskatoon, Canada.
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MeSH Terms
Ammonia / pharmacology*
Astrocytes / drug effects,  enzymology*
Cells, Cultured
Culture Media
Glutamate-Ammonia Ligase / metabolism*
Glutamates / pharmacology*
Glutamic Acid
Glutamine / pharmacology*
Hydrocortisone / pharmacology
Mice, Inbred DBA
Proteins / metabolism
Reg. No./Substance:
0/Culture Media; 0/Glutamates; 0/Proteins; 50-23-7/Hydrocortisone; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; 7664-41-7/Ammonia; EC Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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