Document Detail


Effects of mechanical wounding on Carica papaya cysteine endopeptidases accumulation and activity.
MedLine Citation:
PMID:  16580724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanical wounding impact on the Carica papaya latex protein pattern was investigated by analyzing three latexes. A first one commercially available, a second harvested from unripe but fully grown fruits, both obtained from regularly tapped fruits. A third one was collected from similar fruits but wounded for the first time. The results demonstrated both quantitative and qualitative changes in the protein content and in the enzymatic activity. Repeated wounding results in either, accumulation or activation (or both of them) of papain, chymopapain and caricain. Furthermore, new cysteine protease activity was found to transiently accumulate in the latex collected from newly wounded fruits. The possible implication of this enzymatic material in the papaya cysteine endopeptidases pro-forms activation is discussed.
Authors:
Mohamed Azarkan; Rachid Dibiani; Céline Baulard; Danielle Baeyens-Volant
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Publication Detail:
Type:  Journal Article     Date:  2006-04-03
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  38     ISSN:  0141-8130     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-22     Completed Date:  2006-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  England    
Other Details:
Languages:  eng     Pagination:  216-24     Citation Subset:  IM    
Affiliation:
University of Brussels, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme CP 609, 808, route de Lennik, B-1070 Brussels, Belgium. mazarkan@ulb.ac.be
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MeSH Terms
Descriptor/Qualifier:
Carica / metabolism*
Cations
Chitinase / chemistry
Chromatography
Chromatography, Ion Exchange
Endopeptidases / chemistry*
Fruit
Latex / metabolism
Macromolecular Substances / chemistry
Spectrophotometry
Stress, Mechanical
Chemical
Reg. No./Substance:
0/Cations; 0/Latex; 0/Macromolecular Substances; EC 3.2.1.14/Chitinase; EC 3.4.-/Endopeptidases

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