| Effects of maturation on the phospholipid and phospholipid fatty acid compositions in primary rat cortical astrocyte cell cultures. | |
| | |
MedLine Citation:
|
PMID: 9342724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Phospholipid and phospholipid fatty acid compositional changes were studied in rat cortical astrocytes during dibutyryl cyclic adenosine monophosphate (dBcAMP, 0.25 mM) treatment starting after 14 days in culture (DIC). After 15 DIC, ethanolamine- and choline glycerophospholipid levels were increased 1.2- and 1.3-fold, respectively in treated compared to control cells. However, after 21 and 28 DIC, these levels were not significantly different between groups. Both groups had an increase in phosphatidylserine levels with increasing time in culture. Similarly, ethanolamine plasmalogen levels were transiently elevated after 21 DIC, but returned to previous levels after 28 DIC. The phospholipid fatty acid compositions for the acid stable and labile ethanolamine- and choline glycerophospholipids indicated that in dBcAMP treated cells, 20:4 n-6 and 22:6 n-3 proportions were elevated with increasing time in culture relative to control cells. As 20:4 n-6 proportions increased, there was a concomitant decrease in 20:3 n-9 proportions, suggesting an up regulation of n-6 series elongation and desaturation. In contrast, in control cells, the 20:4 n-6 proportions decreased with a corresponding increase in the 20:3 n-9 proportions. Thus, in treated cells, the cellular phospholipid fatty acid composition was dramatically different than control cells, suggesting that dBcAMP treatment may act to increase fatty acid elongation and desaturation. |
| | |
Authors:
|
E J Murphy; T A Rosenberger; L A Horrocks |
Related Documents
:
|
7059614 - Mitochondrial damage in galactosamine-induced liver intoxication in rats. 1336764 - Influence of chronic ethanol consumption on the inositol phospholipid fatty acid compos... 7929384 - Guanosine tetraphosphate inhibition of fatty acid and phospholipid synthesis in escheri... 6057494 - Positional distribution of isomers of monoenoic fatty acids in animal glycerolipids. 7059614 - Mitochondrial damage in galactosamine-induced liver intoxication in rats. 15164854 - Improved identification of conjugated linoleic acid isomers using silver-ion hplc separ... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Neurochemical research Volume: 22 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1997 Oct |
Date Detail:
|
Created Date: 1998-01-22 Completed Date: 1998-01-22 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1205-13 Citation Subset: IM |
Affiliation:
|
Department of Veterinary Physiology & Pharmacology, Texas A & M University, College Station 77843-4466, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Astrocytes / drug effects, metabolism* Bucladesine / pharmacology Cells, Cultured Cerebral Cortex / cytology, growth & development* Fatty Acids / metabolism* Fatty Acids, Unsaturated / metabolism Phosphatidylcholines / metabolism Phosphatidylinositols / metabolism Phosphatidylserines / metabolism Phospholipids / metabolism* Plasmalogens / metabolism Rats Rats, Sprague-Dawley Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
NS-07291/NS/NINDS NIH HHS; NS-10165/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Phosphatidylcholines; 0/Phosphatidylinositols; 0/Phosphatidylserines; 0/Phospholipids; 0/Plasmalogens; 0/phosphatidal ethanolamines; 362-74-3/Bucladesine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of phosphatidylethanolamine degradation by enzyme(s) of subcellular fractions from cerebr...
Next Document: Lipid-dependent modulation of Ca2+ availability in isolated mossy fiber nerve endings.