Document Detail

Effects of maturation on the phospholipid and phospholipid fatty acid compositions in primary rat cortical astrocyte cell cultures.
MedLine Citation:
PMID:  9342724     Owner:  NLM     Status:  MEDLINE    
Phospholipid and phospholipid fatty acid compositional changes were studied in rat cortical astrocytes during dibutyryl cyclic adenosine monophosphate (dBcAMP, 0.25 mM) treatment starting after 14 days in culture (DIC). After 15 DIC, ethanolamine- and choline glycerophospholipid levels were increased 1.2- and 1.3-fold, respectively in treated compared to control cells. However, after 21 and 28 DIC, these levels were not significantly different between groups. Both groups had an increase in phosphatidylserine levels with increasing time in culture. Similarly, ethanolamine plasmalogen levels were transiently elevated after 21 DIC, but returned to previous levels after 28 DIC. The phospholipid fatty acid compositions for the acid stable and labile ethanolamine- and choline glycerophospholipids indicated that in dBcAMP treated cells, 20:4 n-6 and 22:6 n-3 proportions were elevated with increasing time in culture relative to control cells. As 20:4 n-6 proportions increased, there was a concomitant decrease in 20:3 n-9 proportions, suggesting an up regulation of n-6 series elongation and desaturation. In contrast, in control cells, the 20:4 n-6 proportions decreased with a corresponding increase in the 20:3 n-9 proportions. Thus, in treated cells, the cellular phospholipid fatty acid composition was dramatically different than control cells, suggesting that dBcAMP treatment may act to increase fatty acid elongation and desaturation.
E J Murphy; T A Rosenberger; L A Horrocks
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  22     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1998-01-22     Completed Date:  1998-01-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1205-13     Citation Subset:  IM    
Department of Veterinary Physiology & Pharmacology, Texas A & M University, College Station 77843-4466, USA.
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MeSH Terms
Astrocytes / drug effects,  metabolism*
Bucladesine / pharmacology
Cells, Cultured
Cerebral Cortex / cytology,  growth & development*
Fatty Acids / metabolism*
Fatty Acids, Unsaturated / metabolism
Phosphatidylcholines / metabolism
Phosphatidylinositols / metabolism
Phosphatidylserines / metabolism
Phospholipids / metabolism*
Plasmalogens / metabolism
Rats, Sprague-Dawley
Time Factors
Grant Support
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Phosphatidylcholines; 0/Phosphatidylinositols; 0/Phosphatidylserines; 0/Phospholipids; 0/Plasmalogens; 0/phosphatidal ethanolamines; 362-74-3/Bucladesine

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