| Effects of maternal toluene exposure on testosterone levels in fetal rats. | |
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MedLine Citation:
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PMID: 19110042 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The goal of our study was to determine if toluene affected the synthesis and secretion of testosterone in fetal rats. Dams were exposed to atmospheres that contained 0.09 ppm, 0.9 ppm or 9 ppm of toluene for 90 min/day from gestational days (GDs) 14.5 to 18.5 via nasal inhalation. Fetal plasma testosterone concentrations determined by enzyme immunoassay were significantly reduced on GD 18.5 after exposure to 0.9 and 9 ppm, but not to 0.09 ppm, of toluene in male, but not in female, fetuses. We measured, using real-time PCR methods, mRNA levels in fetal testes for several steroidogenic enzymes involved in testosterone synthesis and insulin-like 3 (Insl3), a maker of Leydig cell differentiation. The mRNA levels of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) were significantly reduced after exposure to 0.9-ppm toluene. However, the mRNA levels of cytochrome P450 cholesterol side-chain cleavage, cytochrome P450 17alpha-hydroxylase/c17-20 lyase, 17beta-hydroxysteroid dehydrogenase, and Insl3 were not significantly altered by exposure to 0.9-ppm toluene. In addition, immunohistochemical analysis showed reduced 3beta-HSD-immunoreactive areas in the interstitial region of fetal testes after exposure to 0.9 and 9 ppm, but not 0.09 ppm, toluene. These findings indicate that toluene reduced the synthesis and secretion of testosterone in fetal testes from rats possibly as a consequence of reduced 3beta-HSD expression. |
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Authors:
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Shinji Tsukahara; Daisuke Nakajima; Yoshiko Kuroda; Rieko Hojo; Shiho Kageyama; Hidekazu Fujimaki |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-06 |
Journal Detail:
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Title: Toxicology letters Volume: 185 ISSN: 0378-4274 ISO Abbreviation: Toxicol. Lett. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-09 Completed Date: 2009-04-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 79-84 Citation Subset: IM |
Affiliation:
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Research Center for Environmental Risk, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan. tsukahara@nies.go.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Air Pollutants
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pharmacokinetics,
toxicity* Animals Enzyme-Linked Immunosorbent Assay Female Fetal Blood / chemistry* Inhalation Exposure / adverse effects* Male Maternal Exposure / adverse effects* Maternal-Fetal Exchange Polymerase Chain Reaction Pregnancy Rats Rats, Long-Evans Testis / drug effects, embryology, metabolism Testosterone / biosynthesis, blood* Toluene / pharmacokinetics, toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Air Pollutants; 108-88-3/Toluene; 58-22-0/Testosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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