| Effects of maternal and dietary selenium (Se-enriched yeast) on oxidative status in testis and apoptosis of germ cells during spermatogenesis of their offspring in goats. | |
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MedLine Citation:
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PMID: 20226605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the effect of maternal and dietary selenium on antioxidant status in testis and apoptosis of germ cells during spermatogenesis of their offspring, selected Taihang Black Goats (n=119) were randomly allotted to four treatment groups. They were fed the experimental diet with different Se levels (from Se-enriched yeast) for 174 d from 60 d prior to lactation to weaning of kids. The treatments were: (1) Group 1 (control), basal diet without Se supplementation, (2) Group 2, the same basal diet supplemented 0.5mg Se/kg DM, (3) Group 3, the same basal diet supplemented 2mg Se/kg DM and (4) Group 4, the same basal diet supplemented 4 mg Se/kg DM. Thirty days after weaning, testis samples of the young male goats were collected for mRNA expression and analyzing the antioxidant status and Se concentration, as well as the population of apoptotic germ cells by TUNEL assay. The results show that mRNA expression of apoptosis genes (Bcl-2, Bax and Caspase 8) were significantly higher in Groups 1 and 4 than that in Groups 2 and 3. The same trend was observed in the population of apoptotic cells analyzed by TUNEL assay. GSH-Px activity and Se concentration in testis of offspring was progressively increased with the increasing Se level in diet of dams. However, there was no significant difference in GSH-Px activity between Groups 3 and 4. The lowest MDA content was obtained in Group 2 and a significant decrease was observed in Groups 1, 3 and 4. These data suggest that doe maternal and dietary Se could influence antioxidant status in the testis of their offspring and the oxidative stress related to Se from the dam could modulate mRNA expression of apoptosis genes and apoptosis of germ cells during spermatogenesis. It is possible that Se supplementation of the dam's diet during gestation and lactation could be a way to supply the Se necessary for normal development of reproductive function of their offspring. |
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Authors:
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Lei Shi; Wenbin Yue; Chunxiang Zhang; Youshe Ren; Xiaomin Zhu; Qian Wang; Liguang Shi; Fulin Lei |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-19 |
Journal Detail:
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Title: Animal reproduction science Volume: 119 ISSN: 1873-2232 ISO Abbreviation: Anim. Reprod. Sci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-03-29 Completed Date: 2010-06-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7807205 Medline TA: Anim Reprod Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 212-8 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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College of Animal Science and Technology, Shanxi Agricultural University, Taigu, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / analysis Apoptosis* / genetics Caspase 3 / genetics Caspase 8 / genetics Colostrum / chemistry Diet Dietary Supplements Female Gene Expression / physiology Genes, bcl-2 / genetics Glutathione Peroxidase / genetics Goats / physiology* In Situ Nick-End Labeling Male Malondialdehyde / analysis Maternal-Fetal Exchange* Milk / chemistry Pregnancy Selenium / administration & dosage*, analysis, blood Spermatogenesis* Spermatozoa / physiology Testis / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 542-78-9/Malondialdehyde; 7782-49-2/Selenium; EC 1.11.1.9/Glutathione Peroxidase; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8 |
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