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[Effects of males' age on sperm apoptosis and DNA integrity].
MedLine Citation:
PMID:  23214251     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVE: To explore the correlation of males'age with sperm apoptosis, sperm DNA integrity and other seminal parameters.
METHODS: We collected 104 semen samples and divided them into three groups according to the males' age: <35 yr (n = 43), 35 -39 yr (n = 31), and > or = 40 yr (n = 30). Based on the WHO Laboratory Manual (4th ed), we detected the seminal parameters, calculated the percentage of apoptotic sperm by flow cytometry (FCM), determined sperm DNA integrity by Acridine orange staining, and compared the results among the three groups.
RESULTS: There were no statistically significant differences among the < 35 yr, 35 -39 yr and > or = 40 yr groups in semen volume ([2.87 +/- 0.89] ml vs [2.98 +/- 1.09] ml vs [2.65 +/- 0.95] ml), sperm concentration ([60.40 +/- 25.43] x 10(6)/ml vs [69.74 +/- 28.33] x 10(6)/ml vs [55.97 +/- 27.22] x 10(6)/ml) (P>0.05). The percentage of progressively motile sperm was significantly lower in the > or = 40 yr ([39.00 +/- 8.35 %) than in the <35 and 35 -39 yr groups ([48.73 +/- 9.89]% and [45.65 +/- 10.55]%) (P<.0.1), and so was the percentage of morphologically normal sperm in the > or = 40 yr than in the < 35 yr group ([11.11 +/- 8.26]% vs [16.43 +/- 8.75 ]%, P<0.01). The percentage of apoptotic sperm was markedly higher in the > or = 40 yr than in the <35 yr group ([11.82 +/- 5.77]% vs [7.04 +/- 3.50]%, P<0.01), while the sperm DNA integrity significantly reduced in the > or = 40 yr group ([75.52 +/- 10.60]%) as compared with the <35 yr ([86.55 +/- 5.60])% and 35 -39 yr group ( [81.39 +/- 8.94]%) (P<0.01). The males' age was correlated positively with the rate of sperm apoptosis (P<0.01), and negatively with sperm DNA integrity and the percentage of progressively motile sperm (P<0.01).
CONCLUSION: The advance in males' age increases sperm apoptosis and reduces sperm progressive motility, normal morphology and DNA integrity.
Authors:
Zhao-Yan Nie; Hai-Feng Wu; Na Zhang; Li-Na Guo; Su-Ying Zhao; Xiu-Li Zhen; Cui-Ting Lü
Publication Detail:
Type:  English Abstract; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zhonghua nan ke xue = National journal of andrology     Volume:  18     ISSN:  1009-3591     ISO Abbreviation:  Zhonghua Nan Ke Xue     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101093592     Medline TA:  Zhonghua Nan Ke Xue     Country:  China    
Other Details:
Languages:  chi     Pagination:  1004-8     Citation Subset:  IM    
Affiliation:
Department of Reproductive Medicine, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China. niezhaoyan@163.com
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