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Effects of magnesium and ionic strength on the diffusion and charge properties of several single tRNA species.
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MedLine Citation:
PMID:  7019856     Owner:  NLM     Status:  MEDLINE    
The technique of laser light scattering was used to evaluate the effects of Mg+2 and ionic strength on the solution structures of seven tRNA species. Information about ion effects on both conformation and electric charge were derived from measurements of the translational diffusion constants and diffusive virial coefficients. E. coli tRNAMetf and six elongator tRNAs from both Class I and II were studied. The diffusion measurements show that the responses of all but the initiator species are qualitatively similar to each other and to that of bulk tRNA, but that significant quantitative differences also obtain. All of the elongator species exhibited an anomolous increase in diffusivity reported earlier by us for bulk tRNA when placed in a low salt-low Mg+2 condition. The initiator tRNA did not undergo this transition and unlike the other tRNAs tested was apparently more compact in 1 mM Mg+2 than 10 mM Mg+2 at ionic strengths in excess of 0.1 M. At 0.1 M ionic strength, pH 7.2, the average net charge of the tRNAs ranged from 7-12 e- in 1 mM Mg+2 and 3-7 e- in 10 mM Mg+2, consistent with the binding of 1-2 additional Mg+2 ions in the higher Mg+2 condition.
K W Rhee; R O Potts; C C Wang; M J Fournier; N C Ford
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  9     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1981 May 
Date Detail:
Created Date:  1981-09-15     Completed Date:  1981-09-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2411-20     Citation Subset:  IM    
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MeSH Terms
Escherichia coli
Magnesium / pharmacology*
Osmolar Concentration
RNA, Transfer / metabolism*
RNA, Transfer, Amino Acyl / metabolism
RNA, Transfer, Met*
Saccharomyces cerevisiae
Reg. No./Substance:
0/RNA, Transfer, Amino Acyl; 0/RNA, Transfer, Met; 0/tRNA, formylmethionine-; 7439-95-4/Magnesium; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 5 Year: 1981
Volume: 9 Issue: 10
First Page: 2411 Last Page: 2420
ID: 326854
PubMed Id: 7019856

Effects of magnesium and ionic strength on the diffusion and charge properties of several single tRNA species.
K W Rhee
R O Potts
C C Wang
M J Fournier
N C Ford, Jr

Article Categories:
  • Research Article

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