| Effects of lymphocyte activation on transfer RNAs. | |
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MedLine Citation:
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PMID: 1953734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influences of mitogen activation on the functional capacity of rat splenic tRNAs were evaluated. The specific amino acid acceptor activity, pmol of a specific amino acid accepted per nmol of tRNA, of isolated splenic tRNAs from in vivo Concanavalin A (37 h)-treated rats were up to 8 times the specific amino acid acceptor activities of splenic tRNAs from control rats. Control splenic tRNAs were treated with purified liver tRNA nucleotidyltransferase in vitro to repair the 3'[CCA] terminus of tRNAs, and subsequently assayed in an aminoacylation reaction. The specific amino acid acceptor activities were slightly increased over those tRNAs not repaired with tRNA nucleotidyltransferase, indicating the presence of a low level of defective but repairable tRNAs in the control rat spleen. Furthermore, our results indicate that cyclosporin A (inhibitor of lymphocyte activation) blocks the Concanavalin A stimulation of tRNA charging ranging from 16 to 93%. |
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Authors:
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K Rasmussen; S Whelly; K Barker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 180 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-12-02 Completed Date: 1991-12-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 638-44 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Texas Tech University Health Sciences Center, Lubbock 79430. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acyl-tRNA Synthetases
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metabolism* Animals Cells, Cultured Concanavalin A DNA Replication* Female Lymphocyte Activation / physiology* Lymphocytes / immunology, physiology* RNA Nucleotidyltransferases / metabolism RNA, Transfer / metabolism* Rats Reference Values Spleen / physiology Thymidine / metabolism |
| Chemical | |
Reg. No./Substance:
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11028-71-0/Concanavalin A; 50-89-5/Thymidine; 9014-25-9/RNA, Transfer; EC 2.7.7.-/RNA Nucleotidyltransferases; EC 2.7.7.-/tRNA nucleotidyltransferase; EC 6.1.1.-/Amino Acyl-tRNA Synthetases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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