Document Detail


Effects of low selenium diets on antioxidant status and MPTP toxicity in mice.
MedLine Citation:
PMID:  1784323     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the role of chronic oxidative stress in MPTP neurotoxicity, C57BL mice were maintained 6-8 weeks on diets deficient in nutrients essential to cellular antioxidant defenses, selenium (Se) and alpha-tocopherol (vit E), and the effects on tissue antioxidant status and MPTP toxicity were evaluated relative to controls on supplemented diets. Activities of the major antioxidant enzymes, glutathione peroxidase (GPx), catalase, and superoxide dismutase, and levels of malondialdehyde as a marker for oxidative stress, were measured in brain, lung, liver and blood. Caudate depletion of dopamine and its metabolites served as a measure of MPTP neurotoxicity. For mice on the Se deficient diet, levels of the selenoenzyme GPx decreased from 50% in brain to 90% in blood. No compensatory changes in the activities of the other antioxidant enzymes were observed and addition of vit E to the diet did not alter antioxidant enzyme activities or malondialdehyde levels. In animals not treated with MPTP, the Se deficient diet significantly increased malondialdehyde only in liver. No protective effect of the antioxidant supplements against caudate depletion of dopamine and its metabolites were observed. However, malondialdehyde levels were increased in the brains of MPTP treated mice on the low Se diets, suggesting the possibility of secondary oxidative damage to tissues accompanying the destruction of substantia nigra neurons by MPTP.
Authors:
M S Sutphin; T D Buckman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  16     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-03-13     Completed Date:  1992-03-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1257-63     Citation Subset:  IM    
Affiliation:
Division of Nutritional Sciences, UCLA School of Public Health 90024.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / pharmacology
Animals
Antioxidants*
Brain / drug effects,  enzymology
Catalase / blood,  metabolism
Caudate Nucleus / drug effects,  metabolism
Diet*
Dopamine / metabolism
Glutathione Peroxidase / blood,  metabolism
Lipid Peroxidation / drug effects
Liver / enzymology
MPTP Poisoning*
Male
Malondialdehyde
Mice
Mice, Inbred C57BL
Selenium / administration & dosage,  deficiency*
Superoxide Dismutase / blood,  metabolism
Vitamin E Deficiency / metabolism
Grant Support
ID/Acronym/Agency:
5 RO1 25797//PHS HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 28289-54-5/1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; 542-78-9/Malondialdehyde; 7782-49-2/Selenium; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

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