Document Detail

Effects of long-term exposure to glucosamine and mannosamine on aggrecan degradation in articular cartilage.
MedLine Citation:
PMID:  12880584     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the effect of long-term exposure to glucosamine or mannosamine on the catabolism of aggrecan by explant cultures of bovine articular cartilage maintained in the presence of retinoic acid. DESIGN: The kinetics of loss of 35S-labeled and total aggrecan from explant cultures of bovine articular cartilage maintained in the presence of 1 micro M retinoic acid and exposed to varying concentrations of glucosamine or mannosamine was investigated over a 9-day culture period. In other experiments, the reversibility of the inhibition of aggrecan catabolism by glucosamine or mannosamine was investigated in cultures exposed to these amino sugars for the first 5 days of a 15-day culture period. The metabolism of chondrocytes exposed to these amino sugars was evaluated by measurement of lactate production or 3H-serine and 35S-sulfate incorporation into protein and glycosaminoglycans, respectively. The direct effect of these amino sugars on soluble aggrecanase activity was determined from immunoblots of aggrecan digests. RESULTS: Glucosamine at 5mM concentration and mannosamine at 2mM concentration inhibited degradation of radiolabeled and chemical levels of aggrecan. At concentrations of up to 10mM amino sugars, the metabolism of chondrocytes was not impaired, as determined by lactate production, protein synthesis and the incorporation of 35S-sulfate into proteoglycans. These amino sugars did not inhibit soluble aggrecanase activity. The exposure of articular cartilage explants to 5mM glucosamine or mannosamine for 5 days in culture in the presence or absence of retinoic acid did not provide long-term suppression of stimulated aggrecan loss. CONCLUSIONS: This study indicates that continuous presence of amino sugars is required to protect cartilage from stimulated loss of aggrecan.
M Z Ilic; B Martinac; C J Handley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society     Volume:  11     ISSN:  1063-4584     ISO Abbreviation:  Osteoarthr. Cartil.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-25     Completed Date:  2003-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9305697     Medline TA:  Osteoarthritis Cartilage     Country:  England    
Other Details:
Languages:  eng     Pagination:  613-22     Citation Subset:  IM    
School of Human Biosciences, La Trobe University, Melbourne, Victoria 3086, Australia.
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MeSH Terms
Blotting, Western / methods
Cartilage, Articular / drug effects*,  metabolism
Culture Techniques / methods
Endopeptidases / metabolism
Extracellular Matrix Proteins*
Glucosamine / pharmacology*
Glycosaminoglycans / analysis
Hexosamines / pharmacology*
Lactates / metabolism
Lectins, C-Type
Protein Biosynthesis
Protein Denaturation / drug effects
Proteoglycans / metabolism*
Reg. No./Substance:
0/Aggrecans; 0/Extracellular Matrix Proteins; 0/Glycosaminoglycans; 0/Hexosamines; 0/Lactates; 0/Lectins, C-Type; 0/Proteoglycans; 2636-92-2/mannosamine; 302-79-4/Tretinoin; 3416-24-8/Glucosamine; EC 3.4.-/Endopeptidases; EC 3.4.99.-/aggrecanase

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