Document Detail


Effects of lipopolysaccharide on interleukin-6 production in perfused human placental cotyledons.
MedLine Citation:
PMID:  11243293     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine if lipopolysaccharide (LPS) alters production of interleukin-6 (IL-6) or vascular tone in perfused placental cotyledons. METHODS: Control and study cotyledons from nine placentas were perfused for 3 h. Study cotyledons received LPS in concentrations of 0.01 mcg/ml (n = 3), 0.1 mcg/ml (n = 3), or 1.0 mcg/ml (n = 3). Effluents were collected at 30, 60, 120, and 180 min following infusion with LPS. IL-6 concentrations were measured by enzyme-linked immunosorbant assay. Perfusion pressures were recorded at 10-min intervals. Data were analyzed using ANOVA for repeated measures. RESULTS: IL-6 production significantly increased over time in both the study and control cotyledons (P = 0.002). LPS treatment did not affect IL-6 production (P = 0.85) and there were no observable dose effects (P = 0.13). Perfusion pressures did not differ (P = 0.16). CONCLUSIONS: The isolated perfused placental cotyledon produces IL-6 and concentrations increase over time. LPS does not alter production of IL-6 or fetoplacental vascular tone.
Authors:
R K Wagner; R M Hinson; C C Apodaca; N Hoeldtke; T Buchanan; R F Hume; B C Calhoun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of maternal-fetal medicine     Volume:  9     ISSN:  1057-0802     ISO Abbreviation:  J Matern Fetal Med     Publication Date:    2000 Nov-Dec
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9211288     Medline TA:  J Matern Fetal Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-5     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Madigan Army Medical Center, Tacoma, Washington, USA. MajWagner@aol.com
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MeSH Terms
Descriptor/Qualifier:
Dose-Response Relationship, Drug
Female
Humans
Interleukin-6 / biosynthesis*
Kinetics
Lipopolysaccharides / administration & dosage,  pharmacology*
Perfusion
Placenta / blood supply*,  drug effects,  metabolism*
Pregnancy
Chemical
Reg. No./Substance:
0/Interleukin-6; 0/Lipopolysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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