| Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats. | |
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MedLine Citation:
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PMID: 21461750 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Lipoic acid (LA) is an antioxidant with therapeutic properties on several diseases like diabetes and obesity. Apelin is a novel adipokine with potential beneficial actions on glucose metabolism and insulin resistance. The aim of this study was to examine in 3T3-L1 adipocytes the effects of LA on apelin gene expression and secretion, as well as elucidate the signaling pathways involved. We also tested the regulation of adipose apelin gene expression by LA supplementation in a model of high-fat diet-induced obesity. LA increased apelin secretion but not apelin gene expression in 3T3-L1 adipocytes. The AMPK inhibitor Compound C induced an increase in LA-stimulated apelin production, and, on the contrary, the AMPK activator AICAR completely reversed the LA stimulatory effects on apelin secretion, also inducing a significant reduction in apelin mRNA levels in this in vitro model. Apelin mRNA levels were increased in those animals fed with the high-fat diet, while the caloric restriction decreased apelin mRNA to control levels. However, apelin gene expression was not significantly modified in rats treated with LA compared with the obese group. The current data suggest the ability of LA to modulate apelin secretion by adipocytes. However the insulin-sensitizing effect of LA in vivo is not related to changes in apelin gene expression in our model of diet-induced obesity. |
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Authors:
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Marta Fernández-Galilea; Patricia Pérez-Matute; Pedro Prieto-Hontoria; J Alfredo Martínez; María Jesús Moreno-Aliaga |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-2 |
Journal Detail:
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Title: Journal of physiology and biochemistry Volume: - ISSN: 1877-8755 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812509 Medline TA: J Physiol Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, C/Irunlarrea 1, 31008, Pamplona, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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