Document Detail


Effects of lipid kinase expression and cellular stimuli on phosphatidylinositol 5-phosphate levels in mammalian cell lines.
MedLine Citation:
PMID:  15876433     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphatidylinositol 5-phosphate (PtdIns5P) is a relatively recently discovered inositol lipid whose metabolism and functions are not yet clearly understood. We have transfected cells with a number of enzymes that are potentially implicated in the synthesis or metabolism of PtdIns5P, or subjected cells to a variety of stimuli, and then measured cellular PtdIns5P levels by a specific mass assay. Stable or transient overexpression of Type IIalpha PtdInsP kinase, or transient overexpression of Type Ialpha or IIbeta PtdInsP kinases caused no significant change in cellular PtdIns5P levels. Similarly, subjecting cells to oxidative stress or EGF stimulation had no significant effect on PtdIns5P, but stimulation of HeLa cells with a phosphoinositide-specific PLC-coupled agonist, histamine, caused a 40% decrease within 1 min. Our data question the degree to which inositide kinases regulate PtdIns5P levels in cells, and we discuss the possibility that a significant part of both the synthesis and removal of this lipid may be regulated by phosphatases and possibly phospholipases.
Authors:
Hilary F Roberts; Jonathan H Clarke; Andrew J Letcher; Robin F Irvine; Katherine A Hinchliffe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  579     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-23     Completed Date:  2005-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2868-72     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Cambridge, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Hela Cells
Humans
Oxidative Stress
Phosphatidylinositol Phosphates / metabolism*
Phosphotransferases / metabolism*
Chemical
Reg. No./Substance:
0/Phosphatidylinositol Phosphates; 0/phosphatidylinositol 5-phosphate; EC 2.7.-/Phosphotransferases

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