Document Detail


Effects of lactose on colon microbial community structure and function in a four-stage semi-continuous culture system.
MedLine Citation:
PMID:  16960357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A semi-continuous four-channel colon simulator was used to study the effects of lactose for the first time on the growth and fermentation dynamics of colonic microbiota. In six separate simulations, lactose supplementation increased the total SCFA concentration by 3-5 fold as compared with the baseline in the respective vessels. The total bacterial density was inversely correlated with lactic acid production (P = 0.003), while production of butyrate (P = 0.007) and propionate (P = 0.02) correlated with higher numbers of bacteria. A major shift in the microbial community structure in the lactose supplemented vessels was demonstrated by bacterial genomic %G+C-profiling of the total population, where lactose supplementation induced a clearly dominant peak in the bifidobacteria prominent area, %G+C 60-65. The transient shift to increased numbers of bifidobacteria (23-27%) of all bacteria in the first two vessels was also confirmed by the bifidobacteria-specific QPCR-method. In conclusion, lactose produced dramatic changes in microbiota composition and activity as compared with the baseline fermentation.
Authors:
Harri A Mäkivuokko; Markku T Saarinen; Arthur C Ouwehand; Nina E Rautonen
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Publication Detail:
Type:  Journal Article     Date:  2006-09-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  70     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-27     Completed Date:  2006-11-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2056-63     Citation Subset:  IM    
Affiliation:
Danisco Innovation, Kantvik, Finland. harri.makivuokko@danisco.com
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MeSH Terms
Descriptor/Qualifier:
Base Composition
Bifidobacterium / genetics,  metabolism*
Biogenic Amines / metabolism
Bioreactors
Colon / drug effects,  microbiology*
DNA, Bacterial / metabolism
Fatty Acids, Volatile / metabolism
Fermentation
Humans
Lactose / metabolism,  pharmacology*
Models, Anatomic
Chemical
Reg. No./Substance:
0/Biogenic Amines; 0/DNA, Bacterial; 0/Fatty Acids, Volatile; 63-42-3/Lactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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