Document Detail

Effects of lactic acid and glycolic acid on human osteoblasts: a way to understand PLGA involvement in PLGA/calcium phosphate composite failure.
MedLine Citation:
PMID:  22105618     Owner:  NLM     Status:  MEDLINE    
The use of degradable composite materials in orthopedics remains a field of intense research due to their ability to support new bone formation and degrade in a controlled manner, broadening their use for orthopedic applications. Poly (lactide-co-glycolide) acid (PLGA), a degradable biopolymer, is now a popular material for different orthopedic applications and is proposed for use in tissue engineering scaffolds either alone or combined with bioactive ceramics. Interference screws composed of calcium phosphates and PLGA are readily available in the market. However, some reports highlight problems of screw migration or aseptic cyst formation following screw degradation. In order to understand these phenomena and to help to improve implant formulation, we have evaluated the effects of PLGA degradation products: lactic acid and glycolic acid on human osteoblasts in vitro. Cell proliferation, differentiation, and matrix mineralization, important for bone healing were studied. It was found that the toxicity of polymer degradation products under buffering conditions was limited to high concentrations. However, non-toxic concentrations led to a decrease in cell proliferation, rapid cell differentiation, and mineralization failure. Calcium, whilst stimulating cell proliferation was not able to overcome the negative effects of high concentrations of lactic and glycolic acids on osteoblasts. These effects help to explain recently reported clinical failures of calcium phosphate/PLGA composites, but further in vitro analyses are needed to mimic the dynamic situation which occurs in the body by, for example, culture of osteoblasts with materials that have been pre-degraded to different extents and thus be able to relate these findings to the degradation studies that have been performed previously.
Florent Meyer; John Wardale; Serena Best; Ruth Cameron; Neil Rushton; Roger Brooks
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-11-21
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  30     ISSN:  1554-527X     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-04-04     Completed Date:  2012-05-22     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  864-71     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Orthopaedic Research Society.
Orthopaedic Research Unit, University of Cambridge, Cambridge, United Kingdom.
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MeSH Terms
Biocompatible Materials
Calcium Phosphates / chemistry*
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Collagen Type I / metabolism
Core Binding Factor Alpha 1 Subunit / genetics,  metabolism
Drug Therapy, Combination
Extracellular Matrix Proteins / metabolism
Gene Expression / drug effects
Glycolates / toxicity*
Lactic Acid / chemistry,  toxicity*
Osteoblasts / drug effects*,  metabolism,  pathology
Osteocalcin / metabolism
Polyglycolic Acid / chemistry*
Prosthesis Design
Prosthesis Failure*
Transcription Factors / genetics,  metabolism
Reg. No./Substance:
0/Biocompatible Materials; 0/Calcium Phosphates; 0/Collagen Type I; 0/Core Binding Factor Alpha 1 Subunit; 0/Extracellular Matrix Proteins; 0/Glycolates; 0/RUNX2 protein, human; 0/Sp7 protein, human; 0/Transcription Factors; 0/polylactic acid-polyglycolic acid copolymer; 104982-03-8/Osteocalcin; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 79-14-1/glycolic acid; 97Z1WI3NDX/calcium phosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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