| Effects of lactic acid and glycolic acid on human osteoblasts: A way to understand PLGA involvement in PLGA/calcium phosphate composite failure. | |
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MedLine Citation:
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PMID: 22105618 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The use of degradable composite materials in orthopedics remains a field of intense research due to their ability to support new bone formation and degrade in a controlled manner, broadening their use for orthopedic applications. Poly (lactide-co-glycolide) acid (PLGA), a degradable biopolymer, is now a popular material for different orthopedic applications and is proposed for use in tissue engineering scaffolds either alone or combined with bioactive ceramics. Interference screws composed of calcium phosphates and PLGA are readily available in the market. However, some reports highlight problems of screw migration or aseptic cyst formation following screw degradation. In order to understand these phenomena and to help to improve implant formulation, we have evaluated the effects of PLGA degradation products: lactic acid and glycolic acid on human osteoblasts in vitro. Cell proliferation, differentiation, and matrix mineralization, important for bone healing were studied. It was found that the toxicity of polymer degradation products under buffering conditions was limited to high concentrations. However, non-toxic concentrations led to a decrease in cell proliferation, rapid cell differentiation, and mineralization failure. Calcium, whilst stimulating cell proliferation was not able to overcome the negative effects of high concentrations of lactic and glycolic acids on osteoblasts. These effects help to explain recently reported clinical failures of calcium phosphate/PLGA composites, but further in vitro analyses are needed to mimic the dynamic situation which occurs in the body by, for example, culture of osteoblasts with materials that have been pre-degraded to different extents and thus be able to relate these findings to the degradation studies that have been performed previously. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. |
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Authors:
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Florent Meyer; John Wardale; Serena Best; Ruth Cameron; Neil Rushton; Roger Brooks |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-21 |
Journal Detail:
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Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: - ISSN: 1554-527X ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Orthopaedic Research Society. |
Affiliation:
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Orthopaedic Research Unit, University of Cambridge, Cambridge, United Kingdom. fmeyer@unistra.fr. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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