| Effects of irradiation on biological behavior of carcinoma cells under carcinoma-stromal cell interaction and air-liquid interface: a possible model for testing radiosensitivity of carcinoma of the upper aerodigestive tract using a collagen gel culture system. | |
| | |
MedLine Citation:
|
PMID: 12234066 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Carcinoma-stromal cell interaction and air-liquid interface (ALI) constitute a specific microenvironment that modulates the biological behavior of carcinoma cells of the upper aerodigestive tract. Although radiotherapy is a useful tool for treating carcinomas of this organ, effects of irradiation on carcinoma cells under carcinoma-stromal cell interaction and ALI are unclear. To address this issue, we examined the effects of irradiation on the proliferation and apoptosis of squamous cell carcinoma cell lines (KB and HEp-2), using three-dimensional collagen gel culture with both carcinoma-stromal cell interaction and ALI. During the second week after irradiation with or without the two factors mentioned above, bromodeoxyuridin (BrdU) uptake and apoptosis of KB, and HEp-2 cell types decreased and increased, respectively. After this stage, the carcinoma cells with these two factors actively re-proliferated together with increased BrdU uptake and decreased apoptosis, whereas the magnitude of these parameters was considerably lower in culture without these factors. We applied our method to carcinoma tissues obtained from several clinical cases. At the same stage, the irradiated carcinoma cells replicated the phenomena observed in cell lines. The data indicate that carcinoma-stromal cell interaction and ALI together promote the re-proliferation of irradiated carcinoma cells and their decreased apoptosis, suggesting that our method is a possible model for testing radiosensitivity of carcinomas in a more physiological condition. |
| | |
Authors:
|
Atsuji Matsuyama; Shuji Toda; Shoichiro Yamada; Akira Inokuchi; Hajime Sugihara |
Related Documents
:
|
16282986 - Phoenix-ampho outperforms pg13 as retroviral packaging cells to transduce human t cells... 8098036 - Regulation of sialomucin production in colon carcinoma cells. 22402126 - Hyperactivation of 4e-binding protein 1 as a mediator of biguanide-induced cytotoxicity... 2120896 - Ultrastructure and neuron-specific enolase (nse) immunohistochemistry of merkel cells i... 9477376 - Muscle satellite cells from dystrophic (mdx) mice have elevated levels of heparan sulph... 1770976 - Quantitative analysis of the purkinje cell population during extreme ageing in the cere... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Pathology, research and practice Volume: 198 ISSN: 0344-0338 ISO Abbreviation: Pathol. Res. Pract. Publication Date: 2002 |
Date Detail:
|
Created Date: 2002-09-17 Completed Date: 2003-03-31 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 7806109 Medline TA: Pathol Res Pract Country: Germany |
Other Details:
|
Languages: eng Pagination: 469-78 Citation Subset: IM |
Affiliation:
|
amatsu@fujita-hu.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adipose Tissue
/
pathology Air Animals Apoptosis / radiation effects* Carcinoma, Squamous Cell / pathology Cell Communication / radiation effects* Cell Culture Techniques / methods* Cell Division / radiation effects* Collagen Fibroblasts / pathology Gels Humans Laryngeal Neoplasms / pathology Mouth Neoplasms / pathology Neoplasm Invasiveness Radiation Tolerance* Rats Stromal Cells / pathology Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Gels; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Apoptosis in thymic epithelial tumors.
Next Document: Focal sarcoid-like change of the thyroid gland. A possible consequence of aspiration cytology?