Document Detail


Effects of intracoronary delivery of allogenic bone marrow-derived stem cells expressing heme oxygenase-1 on myocardial reperfusion injury.
MedLine Citation:
PMID:  22872040     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Heme oxygenase-1 (HO-1) decreases apoptosis, inflammation and oxidative stress. The aim of the study was to investigate the effects of intracoronary infusion of allogenic bone marrow cells (BMC) overexpressing HO-1 in the porcine model of myocardial infarction (MI). MI was produced by balloon occlusion of a coronary artery. BMC were transduced with adenoviruses encoding for HO-1 (HO-1 BMC) or GFP (GFP-BMC) genes. Prior to reperfusion animals received HO-1 BMC, control BMC (unmodified or GFP-BMC) or placebo. Left ventricular (LV) ejection fraction (EF), shortening fraction (SF), end-systolic and end-diastolic diameters (EDD, ESD) were assessed by echocardiography before, 30 minutes (min) and 14 days after reperfusion. BMC significantly improved LVEF and SF early (30 min) after reperfusion as well as after 14 days. Early after reperfusion HO-1 BMC were significantly more effective than control BMC, but after 14 days, there were no differences. There were no effect of cells on LV remodelling and diastolic function. Both HO-1 BMC and control BMC significantly reduced the infarct size vs. placebo (17.2 ± 2.7 and 18.8 ± 2.5, respectively, vs. 27.5 ± 5.1, p= 0.02) in histomorphometry. HO-1-positive donor BMC were detected in the infarct border area in pigs receiving HO-1-cells. No significant differences in expression of inflammatory genes (SDF-1, TNF-α, IL-6, miR21, miR29a and miR133a) in the myocardium were found. In conclusion, intracoronary delivery of allogeneic BMC immediately prior to reperfusion improved the LVEF and reduced the infarct size. HO-1 BMC were not superior to control cells after 14 days however produced faster recovery of LVEF. Transplanted cells survived in the peri-infarct zone.
Authors:
W Wojakowski; M Tendera; W Cybulski; E K Zuba-Surma; K Szade; U Florczyk; M Kozakowska; A Szymula; L Krzych; U Paslawska; R Pasławski; K Milewski; P Buszman; E Nabiałek; W Kuczmik; A Janiszewski; P Dzięgiel; P E Buszman; A Józkowicz; J Dulak
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-07
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  108     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Józef Dulak, PhD, DSc, Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland, Tel.: +48 12 664 6375, E-mail: jozef.dulak@uj.edu.pl or Wojciech Wojakowski, MD, Third Division of Cardiology, Medical University of Silesia, Ziołowa 45, 40-635 Katowice, Poland, Tel.: +48604188669, E-mail: wojtek.wojakowski@gmail.com.
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