Document Detail

Effects of intestinal ischemia-reperfusion on major conduit arteries.
MedLine Citation:
PMID:  10741950     Owner:  NLM     Status:  MEDLINE    
Intestinal ischemia-reperfusion (I-R) is a common and serious clinical condition associated with simultaneous remote organ dysfunction. The purpose of this study was to investigate the effects of intestinal I-R on the vasomotor functions of major conduit arteries. Anesthetized rabbits were randomly assigned to one of three groups: sham-operated controls (Group I), and one-hour intestinal ischemia with two-hour reperfusion (Group II) or four-hour reperfusion (Group III). The following mechanisms of vasomotor functions were studied in abdominal aorta, superior mesenteric, renal, pulmonary, and carotid arterial rings: (1) endothelial-dependent vasodilation response to acetylcholine, (2) endothelial-independent vasodilation response to nitroprusside, (3) beta-adrenergic vasodilation response to isoproterenol, and (4) phenylephrine-induced vasoconstriction. Intestinal injury was quantified using malondialdehyde (MDA) concentration and wet-to-dry intestine weight ratio. Intestinal I-R did not affect the maximal responsiveness or the sensitivity to acetylcholine, nitroprusside, and isoproterenol in all the vessels studied. The maximal contractile response to phenylephrine increased significantly in mesenteric artery in Group II, (227.1+/-15.1% vs. 152.8+/-11.7% in controls) (p<0.05). Intestinal MDA concentration, a marker of oxidant injury, increased from 39.87+/-9.41 nmol/g to 67.8+/-8.8 nmol/g in group II (p<0.01), and to 94.8+/-7.56 nmol/g in Group III (p<0.001). Wet-to-dry intestine weight ratio increased from 3.62+/-0.12 to 4.28+/-0.17 in Group II (p<0.01), to 4.62+/-0.14 in Group III (p<0.001). These data indicate that although the intestines of the animals subjected to intestinal I-R are seriously injured, the smooth muscle relaxation of major conduit arteries was not affected.
C Koksoy; B S Uydes-Dogan; M A Kuzu; A Aydemir-Koksoy; E Demirpence; M Kesenci
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of investigative surgery : the official journal of the Academy of Surgical Research     Volume:  13     ISSN:  0894-1939     ISO Abbreviation:  J Invest Surg     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2000-05-24     Completed Date:  2000-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809255     Medline TA:  J Invest Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  35-43     Citation Subset:  IM    
Department of Surgery, Ankara University Medical School, Turkey.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acetylcholine / pharmacology
Aorta, Abdominal / physiology,  physiopathology
Arteries / drug effects,  physiology,  physiopathology*
Carotid Arteries / physiology
Endothelium, Vascular / physiology
Intestines / blood supply*
Ischemia / physiopathology*
Isoproterenol / pharmacology
Mesenteric Artery, Superior / physiology,  physiopathology
Nitroarginine / pharmacology
Nitroprusside / pharmacology
Phenylephrine / pharmacology
Pulmonary Artery / physiology,  physiopathology
Renal Artery / physiology,  physiopathology
Reperfusion Injury / physiopathology
Thiobarbituric Acid Reactive Substances / analysis
Time Factors
Vasodilation / drug effects
Reg. No./Substance:
0/Thiobarbituric Acid Reactive Substances; 15078-28-1/Nitroprusside; 2149-70-4/Nitroarginine; 51-84-3/Acetylcholine; 59-42-7/Phenylephrine; 7683-59-2/Isoproterenol
Erratum In:
J Invest Surg 2000 Mar-Apr;13(2):125

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Transmyocardial laser revascularization: current status.
Next Document:  A canine model of multiple portosystemic shunting.