Document Detail


Effects of intermediate filaments on actin-based motility of Listeria monocytogenes.
MedLine Citation:
PMID:  11720985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
How does subcellular architecture influence the intracellular movements of large organelles and macromolecular assemblies? To investigate the effects of mechanical changes in cytoplasmic structure on intracellular motility, we have characterized the actin-based motility of the intracellular bacterial pathogen Listeria monocytogenes in normal mouse fibroblasts and in fibroblasts lacking intermediate filaments. The apparent diffusion coefficient of L. monocytogenes was two-fold greater in vimentin-null fibroblasts than in wild-type fibroblasts, indicating that intermediate filaments significantly restrict the Brownian motion of bacteria. However, the mean speed of L. monocytogenes actin-based motility was statistically identical in vimentin-null and wild-type cells. Thus, environmental drag is not rate limiting for bacterial motility. Analysis of the temporal variations in speed measurements indicated that bacteria in vimentin-null cells displayed larger fluctuations in speed than did trajectories in wild-type cells. Similarly, the presence of the vimentin meshwork influenced the turning behavior of the bacteria; in the vimentin-null cells, bacteria made sharper turns than they did in wild-type cells. Taken together, these results suggest that a network of intermediate filaments constrains bacterial movement and operates over distances of several microns to reduce fluctuations in motile behavior.
Authors:
P A Giardini; J A Theriot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  81     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-26     Completed Date:  2002-01-22     Revised Date:  2012-05-17    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3193-203     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / chemistry*
Animals
Cell Movement
Fibroblasts / metabolism,  microbiology
Fluorescent Dyes / pharmacology
Intermediate Filaments / chemistry*,  metabolism*
Kinetics
Listeria monocytogenes / cytology*,  metabolism
Mice
Microscopy, Video
Phalloidine / pharmacology
Rhodamines / pharmacology
Time Factors
Vimentin / chemistry
Grant Support
ID/Acronym/Agency:
CA09302/CA/NCI NIH HHS; R01AI36929/AI/NIAID NIH HHS; R37 AI036929/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Fluorescent Dyes; 0/Rhodamines; 0/Vimentin; 17466-45-4/Phalloidine
Comments/Corrections

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