| Effects of interleukin-1-beta, interleukin-6 and tumor necrosis factor-alpha, alone or in association with hexarelin or galanin, on growth hormone gene expression and growth hormone release from pig pituitary cells. | |
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MedLine Citation:
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PMID: 12324716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: We studied the effects of IL-1beta, IL-6 and TNF-alpha on GH gene expression and secretion with or without galanin and hexarelin. METHODS: Pituitary cells from adult pigs were treated with IL-1beta, IL-6 or TNF-alpha (1, 10 and 100 ng/ml), alone or in association with galanin or hexarelin (10(-8) M): GH mRNA was measured by RT-PCR and GH secretion by ELISA. RESULTS: IL-1beta (1, 10 and 100 ng/ml) and IL-6 (1 and 10 ng/ml) significantly (p < 0.05) enhanced GH output. IL-1beta and TNF-alpha (1 and 10 ng/ml) reduced (p < 0.05) the galanin-induced GH secretion and IL-6 (10 ng/ml) potentiated the effect of both GH releasers (p < 0.05). GH gene expression was increased only by IL-6 at the concentrations of 1 and 10 ng/ml, either alone or in association with both galanin and hexarelin. CONCLUSIONS: We hypothesize that cytokines may play a paracrine/autocrine role in GH regulation in the pituitary independently from the intracellular pathways of the GH secretagogues. |
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Authors:
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Gian Luca Mainardi; Roberta Saleri; Carlo Tamanini; Mario Baratta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hormone research Volume: 58 ISSN: 0301-0163 ISO Abbreviation: Horm. Res. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-09-26 Completed Date: 2003-03-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0366126 Medline TA: Horm Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 180-6 Citation Subset: IM |
Copyright Information:
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Copyright 2002 S. Karger AG, Basel |
Affiliation:
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Department of Animal Production, Veterinary Biotechnology and Food Safety, Section of Veterinary Physiology, University of Parma, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cytokines / pharmacology* Female Galanin / pharmacology* Gene Expression / drug effects Growth Hormone / genetics*, secretion* Interleukin-1 / pharmacology Interleukin-6 / pharmacology Oligopeptides / pharmacology* Pituitary Gland / drug effects, metabolism* RNA, Messenger / analysis Swine Time Factors Tumor Necrosis Factor-alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Interleukin-1; 0/Interleukin-6; 0/Oligopeptides; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 140703-51-1/hexarelin; 88813-36-9/Galanin; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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