Document Detail


Effects of the interaction between beta-carboline-3-carboxylic acid N-methylamide and polynucleotides on singlet oxygen quantum yield and DNA oxidative damage.
MedLine Citation:
PMID:  18028221     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The complexation of beta-carboline-3-carboxylic acid N-methylamide (betaCMAM) with the sodium salts of the nucleotides polyadenylic (Poly A), polycytidylic (Poly C), polyguanylic (Poly G), polythymidylic (Poly T) and polyuridylic (Poly U) acids, and with double stranded (dsDNA) and single stranded deoxyribonucleic acids (ssDNA) was studied at pH 4, 6 and 9. Predominant 1:1 complex formation is indicated from Job plots. Association constants were determined using the Benesi-Hildebrand equation. BetaCMAM-sensitized singlet oxygen quantum yields were determined at pH 4, 6 and 9, and the effects on this of adding oligonucleotides, dsDNA and ssDNA were studied at the three pH values. With dsDNA, the effect on betaCMAM triplet state formation was also determined through triplet-triplet transient absorption spectra. To evaluate possible oxidative damage of DNA following singlet oxygen betaCMAM photosensitization, we used thiobarbituric acid-reactivity assays and electrophoretic separation of DNA assays. The results showed no oxidative damage at the level of DNA degradation or strand break.
Authors:
Iñigo X García-Zubiri; Hugh D Burrows; J Sérgio Seixas de Melo; Joao Pina; María Monteserín; María J Tapia
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  83     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2007-11-21     Completed Date:  2008-02-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1455-64     Citation Subset:  IM    
Affiliation:
Departamento de Química, Universidad de Burgos, Burgos, Spain.
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MeSH Terms
Descriptor/Qualifier:
Carbolines / chemistry*
DNA Damage / genetics*
Molecular Structure
Oxidation-Reduction
Polynucleotides / chemistry*,  genetics*
Singlet Oxygen / chemistry*
Spectrophotometry
Chemical
Reg. No./Substance:
0/Carbolines; 0/Polynucleotides; 0/beta-carboline-3-carboxylic acid N-methylamide; 17778-80-2/Singlet Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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