| Effects of insulin and insulin-like growth factor I on growth of human leukemia cells in serum-free and protein-free medium. | |
| | |
MedLine Citation:
|
PMID: 3291986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human myeloid leukemia cells (HL60) and malignant lymphocytes (Namalwa) were grown in protein-free, Fe-supplemented media and used to study growth responses to insulin and insulin-like growth factor 1 (IGF-I). HL60 cells previously grown in serum-free medium containing microgram quantities of insulin showed an 18-fold reduction in cumulative cell production when grown without insulin. However, the same cells showed reduced or absent growth stimulation with 1 to 100 ng/mL insulin or IGF-I for at least four days following insulin deprivation, indicating that culture conditions modified insulin and IGF-I responses. When the same cells were grown in Fe-supplemented, protein-free medium (RPMI-Fe), insulin and IGF-I caused dose-dependent stimulation of HL60 cell growth with half-maximal stimulation at nanogram concentrations. Namalwa cells grown in protein-free medium showed no response to either hormone. Radioligand binding showed the presence of insulin and IGF-I receptors on both HL60 and Namalwa cells grown in RPMI-Fe. HL60 cells grown in fetal bovine serum had higher, and cells grown with microgram quantities of insulin dramatically reduced, insulin binding. Competitive binding studies and cultures with anti-IGF-I receptor antibody showed insulin and IGF-I stimulated growth through their respective specific receptors. Both insulin and IGF-I stimulate growth of some cultured human leukemia cells, but the presence of insulin or IGF-I receptors alone does not predict growth responses. Culture conditions affect both cellular responses and ligand binding by these hormones and must be closely controlled to study growth responses. |
| | |
Authors:
|
J Sinclair; D McClain; R Taetle |
Related Documents
:
|
10526246 - Effect of 6 months of gh treatment on myosin heavy chain composition in gh-deficient pa... 8491986 - Epidermal growth factor and insulin-like growth factor i enhance keratinocyte migration. 21425726 - Prevalence and effect of hemoglobin e disorders on hba1c and lipid profile of diabetic ... 15812646 - The effect of hypophysectomy on pancreatic islet hormone and insulin-like growth factor... 12702046 - Tropical sprue is associated with contamination of small bowel with aerobic bacteria an... 23550246 - Complex type 2 diabetes mellitus - management challenges and pitfalls. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Blood Volume: 72 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1988 Jul |
Date Detail:
|
Created Date: 1988-08-19 Completed Date: 1988-08-19 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 66-72 Citation Subset: AIM; IM |
Affiliation:
|
Department of Medicine, University of California, San Diego 92103. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies, Monoclonal
/
physiology Binding, Competitive Cell Division / drug effects* Cell Line Culture Media / analysis* Dose-Response Relationship, Drug Fetal Blood Humans Insulin / metabolism, pharmacology* Insulin-Like Growth Factor I / metabolism, pharmacology* Leukemia / metabolism, pathology* Proteins Receptor, Insulin / analysis, drug effects, immunology Somatomedins / pharmacology* Time Factors Tumor Cells, Cultured / metabolism, pathology* |
| Grant Support | |
ID/Acronym/Agency:
|
CA09290/CA/NCI NIH HHS; CA23100/CA/NCI NIH HHS; CA32094/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Culture Media; 0/Proteins; 0/Somatomedins; 11061-68-0/Insulin; 67763-96-6/Insulin-Like Growth Factor I; EC 2.7.10.1/Receptor, Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Antimalarial properties of orally active iron chelators.
Next Document: Partial chimerism after T-cell-depleted allogeneic bone marrow transplantation in leukemic HLA-match...