| Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro. | |
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MedLine Citation:
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PMID: 22205616 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5-20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone. |
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Authors:
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Yu-Li Huang; Ruo-Feng Qiu; Wei-Yi Mai; Jian Kuang; Xiao-Yan Cai; Yu-Gang Dong; Yun-Zhao Hu; Yuan-Bin Song; An-Ping Cai; Zhi-Gao Jiang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of Zhejiang University. Science. B Volume: 13 ISSN: 1862-1783 ISO Abbreviation: J Zhejiang Univ Sci B Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2011-12-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101236535 Medline TA: J Zhejiang Univ Sci B Country: China |
Other Details:
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Languages: eng Pagination: 20-8 Citation Subset: IM |
Affiliation:
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Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China; Department of Cardiology, the First People's Hospital of Shunde, Foshan 528300, China. |
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