Document Detail


Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro.
MedLine Citation:
PMID:  22205616     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs).
METHODS: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5-20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA.
CONCLUSIONS: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.
Authors:
Yu-li Huang; Ruo-feng Qiu; Wei-yi Mai; Jian Kuang; Xiao-yan Cai; Yu-gang Dong; Yun-zhao Hu; Yuan-bin Song; An-ping Cai; Zhi-gao Jiang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  13     ISSN:  1862-1783     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-29     Completed Date:  2012-04-26     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  20-8     Citation Subset:  IM    
Affiliation:
Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / drug effects,  physiology
Cell Movement / drug effects,  physiology
Collagen Type II / genetics,  metabolism
Dose-Response Relationship, Drug
Flow Cytometry
GATA4 Transcription Factor / genetics,  metabolism
Insulin-Like Growth Factor I / metabolism,  pharmacology*,  physiology
Mesenchymal Stromal Cells / cytology,  drug effects*,  metabolism
RNA, Messenger / chemistry,  genetics
Rats
Rats, Sprague-Dawley
Receptors, CXCR4 / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Collagen Type II; 0/GATA4 Transcription Factor; 0/RNA, Messenger; 0/Receptors, CXCR4; 67763-96-6/Insulin-Like Growth Factor I
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