Document Detail

Effects of insulin and exercise on rat hindlimb muscles after simulated microgravity.
MedLine Citation:
PMID:  1474084     Owner:  NLM     Status:  MEDLINE    
This study was designed to examine insulin- and exercise-stimulated glucose uptake and metabolism in the hindlimb muscles of rats after conditions of simulated microgravity. To simulate microgravity, male Sprague-Dawley rats were suspended in a head-down (45 degrees) position with their hindlimbs non-weight bearing (SUS) for 14 days. In addition, rats were assigned to suspension followed by exercise (SUS-E), to cage control (CC), or to exercising control (CC-E) groups. Exercise consisted of five 10-min bouts of treadmill running at the same relative intensity for the CC-E and SUS-E rats (80-90% of maximum O2 consumption). Hindlimb perfusion results indicated that glucose uptake for the entire hindquarter at 24,000 microU/ml insulin (maximum stimulation) was significantly higher in the SUS (8.9 +/- 0.5 mumol.g-1.h-1) than in the CC (7.6 +/- 0.4 mumol.g-1.h-1) rats, signifying an increased insulin responsiveness. Glucose uptake at 90 microU/ml insulin was also significantly higher in the SUS (48 +/- 4; % of maximum stimulation over basal) than in the CC (21 +/- 4%) rats. In addition, exercise-induced increases in glucose uptake for the hindlimbs (133%) and glucose incorporation into glycogen for the plantaris (8.4-fold), extensor digitorum longus (5.4-fold), and white gastrocnemius (4.8-fold) muscles were greater for the SUS-E rats than for the CC-E rats (39% and 1.9-, 1.9-, and 3.0-fold, respectively). Therefore, suspension of the rat with hindlimbs non-weight bearing leads to enhanced muscle responses to insulin and exercise when they were applied separately. However, insulin action appeared to be impaired after exercise for the SUS-E rats, especially for the soleus muscle.
C S Stump; T W Balon; C M Tipton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  73     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-02-01     Completed Date:  1993-02-01     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2044-53     Citation Subset:  IM; S    
Department of Exercise and Sport Sciences, School of Health Related Professions, University of Arizona, Tucson 85721.
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MeSH Terms
Body Weight / physiology
Carbon Dioxide / metabolism
Drinking / physiology
Eating / physiology
Glucose / metabolism
Glucosephosphates / metabolism
Glycogen / metabolism
Hindlimb / blood supply,  physiology*
Insulin / pharmacology*
Lactates / metabolism
Lactic Acid
Muscles / physiology*
Oxygen Consumption / physiology
Physical Exertion / physiology*
Pyruvates / metabolism
Pyruvic Acid
Rats, Sprague-Dawley
Regional Blood Flow / physiology
Weightlessness / adverse effects*
Reg. No./Substance:
0/Glucosephosphates; 0/Insulin; 0/Lactates; 0/Pyruvates; 124-38-9/Carbon Dioxide; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 50-99-7/Glucose; 9005-79-2/Glycogen
C M Tipton / U AZ, Tucson

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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