Document Detail


Effects of infrared laser damage to the Euglena photoreceptor on the control of flagellar motility.
MedLine Citation:
PMID:  7168846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When the area of the stigma of Euglena was irradiated with an infrared laser beam at a dose too low to cause permanent loss of motility, a reduction in flagellar motility was observed only when the external medium contained less than 1 mM Mg2+. At these low Mg2+ concentrations, the laser caused a decrease in flagellar frequency and a tendency for the flagellar waveform to shift towards that taken during reversed swimming. This suggests that the effect of the laser irradiation was to deplete the cells of Mg2+. After the laser pulse the reversal response remained sensitive to the wavelength of the illuminating light. In white light (420-700 nm) 60% of the Euglena showed a reversed waveform; in orange light (530-700 nm) this increased to 90%. This shows that the photoreceptor was not destroyed by the laser irradiation. These experiments were performed on cells that had been impaled on a microelectrode. If direct electric current was passed into the laser-irradiated cells, the current necessary to cause flagellar arrest was 2 to 4 times less than that for cells not laser irradiated. It is concluded that an internal Mg2+ store is present in the Euglena, localized in the area of the paraflagellar swelling; and that the laser irradiation eliminates this Mg2+ store, but at the power used it does not destroy the ability of the stigma-paraflagella to control the flagellar activity.
Authors:
K M Nichols; R Rikmenspoel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell motility     Volume:  2     ISSN:  0271-6585     ISO Abbreviation:  Cell Motil.     Publication Date:  1982  
Date Detail:
Created Date:  1983-06-17     Completed Date:  1983-06-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8207421     Medline TA:  Cell Motil     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  573-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / physiology
Calcium / physiology
Euglena / physiology*
Flagella / physiology*
Lasers
Magnesium / physiology
Photoreceptor Cells / radiation effects
Grant Support
ID/Acronym/Agency:
HD6445/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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