| Effects of infant formula on cell homeostasis and cytokine levels in an in vivo and in vitro murine aspiration model. | |
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MedLine Citation:
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PMID: 21520431 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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RATIONALE: The role of infant formula aspiration in lung injury has not been studied extensively. We evaluated the effects of a single infant formula aspiration into the lungs of mice and the effect of infant formula exposure on cell lines representing murine alveolar macrophages and type II epithelial cells. OBJECTIVES: To study the effects of exposure to infant formula on cell count histology and cytokine levels in an in vivo and in vitro model of aspiration. METHODS: In vivo: Juvenile mice received 2.5 µl/g of 50% infant formula intranasally. Bronchoalveolar lavage samples were collected at 1, 2, and 7 days after aspiration and evaluated for cell count and differential. In vitro: RAW 264.7 and MLE-15 cells were exposed to 1% infant formula for 6 hr. Extracellular levels of IL-6, TNF-α, MIP-2, and KC were measured in lavage fluid and cell media using ELISA assays. RESULTS: In vivo: An increase in neutrophils, IL-6 and KC levels were noted 24 hr after infant formula exposure. In vitro: An increase in TNF-α levels from RAW 264.7 and MIP-2 and KC levels from MLE-15 cells was noted after infant formula exposure. CONCLUSIONS: A single aspiration of infant formula into the lungs leads to an acute inflammatory response involving both lung macrophages and epithelial cells. Pediatr. Pulmonol. © 2011 Wiley-Liss, Inc. |
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Authors:
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Derek L Pepiak; Joseph L Alcorn; Constance L Atkins; Hasen Xue; Giuseppe N Colasurdo; Amir M Khan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-25 |
Journal Detail:
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Title: Pediatric pulmonology Volume: - ISSN: 1099-0496 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8510590 Medline TA: Pediatr Pulmonol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Departments of Pediatrics and Surgery, University of Texas, Houston Medical School, Houston, Texas. dpepia@lsuhsc.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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