Document Detail

Effects of infant formula on cell homeostasis and cytokine levels in an in vivo and in vitro murine aspiration model.
MedLine Citation:
PMID:  21520431     Owner:  NLM     Status:  Publisher    
RATIONALE: The role of infant formula aspiration in lung injury has not been studied extensively. We evaluated the effects of a single infant formula aspiration into the lungs of mice and the effect of infant formula exposure on cell lines representing murine alveolar macrophages and type II epithelial cells. OBJECTIVES: To study the effects of exposure to infant formula on cell count histology and cytokine levels in an in vivo and in vitro model of aspiration. METHODS: In vivo: Juvenile mice received 2.5 µl/g of 50% infant formula intranasally. Bronchoalveolar lavage samples were collected at 1, 2, and 7 days after aspiration and evaluated for cell count and differential. In vitro: RAW 264.7 and MLE-15 cells were exposed to 1% infant formula for 6 hr. Extracellular levels of IL-6, TNF-α, MIP-2, and KC were measured in lavage fluid and cell media using ELISA assays. RESULTS: In vivo: An increase in neutrophils, IL-6 and KC levels were noted 24 hr after infant formula exposure. In vitro: An increase in TNF-α levels from RAW 264.7 and MIP-2 and KC levels from MLE-15 cells was noted after infant formula exposure. CONCLUSIONS: A single aspiration of infant formula into the lungs leads to an acute inflammatory response involving both lung macrophages and epithelial cells. Pediatr. Pulmonol. © 2011 Wiley-Liss, Inc.
Derek L Pepiak; Joseph L Alcorn; Constance L Atkins; Hasen Xue; Giuseppe N Colasurdo; Amir M Khan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-25
Journal Detail:
Title:  Pediatric pulmonology     Volume:  -     ISSN:  1099-0496     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510590     Medline TA:  Pediatr Pulmonol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Departments of Pediatrics and Surgery, University of Texas, Houston Medical School, Houston, Texas.
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