Document Detail

Effects of indomethacin, luteinizing hormone (LH), prostaglandin E(2) (PGE(2)), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F(2alpha) (PGF(2alpha)) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle.
MedLine Citation:
PMID:  11278017     Owner:  NLM     Status:  Publisher    
Two experiments were conducted to determine the luteotropin of pregnancy in sheep and to examine autocrine and paracrine roles of progesterone and estradiol-17 beta on progesterone secretion by the ovine corpus luteum (CL). Secretion of progesterone per unit mass by day-8 or day-11 CL of the estrous cycle was similar to day-90 CL of pregnancy (P >/= 0.05). In experiment 1, secretion of progesterone in vitro by slices of CL from ewes on day-8 of the estrous cycle was increased (P </= 0.05) by LH or PGE(2). Secretion of progesterone in vitro by CL slices from day-90 pregnant ewes was not affected by LH (P >/= 0.05) while PGE(2) increased (P </= 0.05) secretion of progesterone. Day 8 ovine CL of the estrous cycle did not secrete (P >/= 0.05) detectable quantities of PGF(2alpha) or PGE while day-90 ovine CL of pregnancy secreted PGE (P </= 0.05) but not PGF(2alpha). Secretion of progesterone and PGE in vitro by day-90 CL of pregnancy was decreased (P </= 0.05) by indomethacin. The addition of PGE(2), but not LH, in combination with indomethacin overcame the decreases in progesterone by indomethacin (P </= 0.05).In experiment 2, secretion of progesterone in vitro by day-11 CL of the estrous cycle was increased at 4-h (P </= 0.05) in the absence of treatments. Both day-11 CL of the estrous cycle and day-90 CL of pregnancy secreted detectable quantities of PGE and PGF(2alpha) (P </= 0.05). In experiment 1, PGF(2alpha) secretion by day-8 CL of the estrous cycle and day-90 ovine CL of pregnancy was undetectable, but was detectable in experiment 2 by day-90 CL. Day 90 ovine CL of pregnancy also secreted more PGE than day-11 CL of the estrous cycle (P </= 0.05), whereas day-8 CL of the estrous cycle did not secrete detectable quantities of PGE (P >/= 0.05). Trilostane, mifepristone, or MER-25 did not affect secretion of progesterone, PGE, or PGF(2alpha) by day-11 CL of the estrous cycle or day-90 CL of pregnancy (P >/= 0.05). It is concluded that PGE(2), not LH, is the luteotropin at day-90 of pregnancy in sheep and that progesterone does not modify the response to luteotropins. Thus, we found no evidence for an autocrine or paracrine role for progesterone or estradiol-17 36 on luteal secretion of progesterone, PGE or PGF(2alpha).
L Kim; Y S. Weems; P J. Bridges; B R. LeaMaster; L Ching; D L. Vincent; C W. Weems
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Publication Detail:
Journal Detail:
Title:  Prostaglandins     Volume:  63     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-Mar-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  -    
Other Details:
Languages:  ENG     Pagination:  189-203     Citation Subset:  -    
Dept. of Animal Science, University of Hawaii, 96822, Honolulu, HI, USA
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