Document Detail


Effects of increased pulmonary flow on the expression of endothelial nitric oxide synthase and endothelin-1 in the rat.
MedLine Citation:
PMID:  12193106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of the study was to assess whether increased pulmonary flow and subsequent development of pulmonary vascular remodelling could alter the expression of endothelial nitric oxide synthase (eNOS) and endothelin-1 (ET-1) in the rat lung. Nine 42-day-old Wistar rats underwent abdominal aortocaval shunt to increase pulmonary blood flow for 12 weeks. The shunt resulted in significant medial hypertrophy of pulmonary artery without significant alterations in pulmonary or systemic blood pressure. Using competitive reverse transcription-PCR, significant increases in the preproET-1 mRNA expression and eNOS mRNA expression in the lungs of rats with abdominal aortocaval shunt were detected. Increased eNOS protein in the lung of shunt rats was also found by Western blot analysis. However, the plasma ET-1 concentration in the pulmonary artery (sham: 5+/-0.7 pg/ml; shunt: 6+/-0.8 pg/ml) or the lung ET-1 content (sham: 218+/-41 ng/g protein; shunt: 224+/-40 ng/g protein) was unchanged. There was an elevated immunohistochemical expression of eNOS, but not ET-1, in the pulmonary vascular endothelium in rats with the shunt. These results suggest that eNOS and ET-1 may be involved in remodelling prior to the development of pulmonary hypertension.
Authors:
Zen-Kong Dai; Mian-Shin Tan; Chee-Yin Chai; Ing-Jun Chen; Arco Y Jeng; Jiunn-Ren Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  103 Suppl 48     ISSN:  0143-5221     ISO Abbreviation:  Clin. Sci.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-23     Completed Date:  2002-11-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  289S-293S     Citation Subset:  IM    
Affiliation:
Division of Cardiology and Pulmonology, Department of Pediatrics, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Sun-Ming District, Kaohsiung, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Endothelin-1 / metabolism*
Endothelins / genetics
Endothelium, Vascular / chemistry,  metabolism*
Hypertension, Pulmonary / metabolism*,  pathology
Hypertrophy
Immunohistochemistry / methods
Lung / metabolism*,  pathology
Models, Animal
Nitric Oxide Synthase / metabolism*
Nitric Oxide Synthase Type III
Protein Precursors / genetics
Pulmonary Artery / pathology
RNA, Messenger / analysis
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Endothelin-1; 0/Endothelins; 0/Protein Precursors; 0/RNA, Messenger; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, rat

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