| Effects of icariin on improving erectile function in streptozotocin-induced diabetic rats. | |
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MedLine Citation:
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PMID: 21967314 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Introduction. Icariin has been shown to improve penile hemodynamics in animal models of erectile dysfunction from cavernous nerve injury and castration. The effects of icariin on penile hemodynamics in diabetic animals remain to be determined. Transforming growth factor β1 (TGFβ1) has been implicated in the pathogenesis of diabetes-related erectile dysfunction. Aim. The aim of this study was to investigate the effects of icariin in the penis of streptozotocin (STZ)-induced diabetic rat. Methods. Two-month-old Sprague-Dawley male rats received one-time intraperitoneal (IP) STZ (60 mg/kg) or vehicle injection after a 16-hour fast. Three days later, the STZ-induced diabetic rats were randomly divided into four groups and were treated with daily gavage feedings of a 50:50 mix of normal saline and dimethyl sulfoxide (DMSO) or icariin dissolved in DMSO at doses of 1, 5, and 10 mg/kg for 3 months. A positive control group underwent IP injection of saline followed by daily gavage of saline/DMSO solution. Treatment was stopped 1 week prior to functional assay and euthanasia. Main Outcome Measure. Penile hemodynamics was assessed by electrical stimulation of the cavernous nerves with real-time intracavernous pressure (ICP) measurement. After euthanasia, penile tissue was studied using immunohistochemistry, Western blot, and enzyme-linked immunosorbent assay (ELISA) to assess the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) and TGFβ1/Smad2 signaling pathway. Results. Diabetes attenuated ICP response in control animals. Untreated diabetic animals had decreased smooth muscle/collagen ratio and endothelial cell content in the corpora cavernosa; treatment with icariin partially attenuating these effects. Icariin-treated animals also had a significantly greater expression of nicotinamide adenine dinucleotide phosphate-positive nerves and the endothelial cell markers, von Willebrand factor (vWF), and platelet endothelial cell adhesion molecule-1 (PECAM). TGFβ1/Smad2 signaling pathway was down-regulated in the penis from icariin-treated models relative to what was observed in negative control animals. Conclusion. Icariin treatment preserved penile hemodynamics, smooth muscle and endothelial integrity, and neuronal nitric oxide synthase expression in the penis of diabetic rats. Down-regulation of TGFβ1/Smad2 signaling pathway might mediate this effect. Liu T, Xin H, Li W-R, Zhou F, Li G-Y, Gong Y-Q, Gao Z-Z, Qin X-C, Cui W-S, Shindel AW, and Xin Z-C. Effects of icariin on improving erectile function in streptozotocin-induced diabetic rats. J Sex Med 2011;8:2761-2772. |
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Authors:
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Tao Liu; Hua Xin; Wei-Ren Li; Feng Zhou; Guang-Yong Li; Yan-Qing Gong; Zhe-Zhu Gao; Xin-Cheng Qin; Wan-Shou Cui; Alan W Shindel; Zhong-Cheng Xin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The journal of sexual medicine Volume: 8 ISSN: 1743-6109 ISO Abbreviation: J Sex Med Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101230693 Medline TA: J Sex Med Country: United States |
Other Details:
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Languages: eng Pagination: 2761-72 Citation Subset: IM |
Copyright Information:
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© 2011 International Society for Sexual Medicine. |
Affiliation:
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Andrology Center, Peking University First Hospital, Beijing, China Department of Ophthalmology, Chonbuk National University Hospital, Jeonju, Korea National Institute for Communicable Disease Control and Prevention, Chinese Disease Control and Prevention Center, Beijing, China Department of Urology, University of California at Davis, Sacramento, CA, USA. |
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