| Effects of hyperthermia on rat hippocampal pyramidal cell apoptosis in vitro. | |
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MedLine Citation:
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PMID: 12651238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the changes in the morphology and biochemistry of neonatal rat hippocampal pyramidal cells (HPCs)apoptosis in high-temperature environment. METHODS: In vitro culture of HPCs was established and then subjected to environmental stimulation with high temperatures (37, 40, and 42 degrees Celsius respectively) for 1 h. All the cells were collected at different time points after the treatment and examined by flow cytometery, fluorescence staining, agarose gel electrophoresis and in situ cell apoptosis detection kit. RESULTS: The apoptotic rate and the number of apoptotic bodies increased as the temperature was elevated. DNA ladder was presented on agarose gel electrophoresis, and the germination of the apoptotic body was observed by fluorescence microscope. CONCLUSIONS: Hyperthermia may induce the apoptosis in HPCs in vitro, and the extend of apoptosis and apoptotic behavior may vary with the temperature and treatment duration. |
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Authors:
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Guang-zZhong Chen; Bing-dDe Luo; Hong-qQin Wang; Fei Zou; Wei-rRen Wan; Xiao-hHong Shen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Volume: 23 ISSN: 1000-2588 ISO Abbreviation: Di Yi Jun Yi Da Xue Xue Bao Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-24 Completed Date: 2003-12-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9426110 Medline TA: Di Yi Jun Yi Da Xue Xue Bao Country: China |
Other Details:
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Languages: eng Pagination: 233-5 Citation Subset: IM |
Affiliation:
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Department of High-temperature Medicine, First Military Medical University, Guangzhou 510515, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Body Temperature Cells, Cultured Female Hippocampus / cytology* Hyperthermia, Induced / adverse effects* Male Pyramidal Cells / cytology* Rats Rats, Sprague-Dawley |
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