| Effects of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel blockers on the proliferation and cell cycle progression of embryonic stem cells. | |
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MedLine Citation:
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PMID: 21110038 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Embryonic stem cells (ESCs) can uniquely proliferate indefinitely and differentiate into all cell lineages. ESCs may therefore provide an unlimited supply of cells for cell-based therapies. Previous study reported the presence of hyperpolarization-activated inward currents in undifferentiated mouse (m) ESCs, but the functional role of this hyperpolarization-activated current in mESCs is unknown. In this study, the role of this current in maintaining the proliferative capacity and the cell cycle progression of ESCs was investigated. In D3 mESCs, this hyperpolarization-activated inward current can be blocked by HCN channel blocker ZD7288. Application of the HCN channel blockers, cesium (1-10 mM) or ZD7288 (0.1-30 μM), attenuated cell proliferation in a concentration-dependent manner. Both HCN blockers were found to be non-cytotoxic to mESCs as determined by cell viability test. Interestingly, ZD7288 at 10 and 30 μM was found to decrease the proportion of cells in G(0)/G(1) phase and increase the proportion of cells in S phase. This suggests that this hyperpolarization-activated current can affect the cell cycle progression in mESCs. In summary, the present investigation suggests that ESC proliferation and cell cycle progression can be regulated by this hyperpolarization-activated current. |
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Authors:
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Yuen-Ting Lau; Chun-Kit Wong; Jialie Luo; Lok-Hang Leung; Pui-Fong Tsang; Zhao-Xiang Bian; Suk-Ying Tsang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-26 |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 461 ISSN: 1432-2013 ISO Abbreviation: Pflugers Arch. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: Germany |
Other Details:
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Languages: eng Pagination: 191-202 Citation Subset: IM |
Affiliation:
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Biochemistry Program, School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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