| Effects of hypercholesterolemia on the microsomal membrane fluidity of intimal-medial versus medial layers of swine aorta: implications for the pathogenesis of vasospasm. | |
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MedLine Citation:
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PMID: 3609245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hypothesis was examined that hypercholesterolemia induces a decrease in arterial microsomal membrane fluidity. To investigate this hypothesis, the fluorescence anisotropy (r) of 1,6-diphenylhexa-1,3,5-triene was measured in aortic microsomes isolated from the intimal-medial (IM) and medial (M) layers of swine thoracic aortas. After 10 weeks of feeding a high fat (10% lard) diet, serum cholesterol increased 2.3-fold compared to 3.6-fold in pigs fed a similar diet supplemented with 2% cholesterol. Based upon differences in r, the membrane fluidity of the IM layer was significantly less than the M layers. The membrane fluidity of the IM layer was inversely related to the severity of hypercholesterolemia regardless of dietary treatment. There were no differences in membrane fluidity among the three dissected M layers and the membrane fluidity of these layers was refractory to changes in serum cholesterol. A decrease in the membrane fluidity of the IM layer may contribute to the abnormal regulation of vascular tone which underlies the development of vasospasm in atherosclerotic arteries. |
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Authors:
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P Gillies; C Robinson; R Chapple |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental and molecular pathology Volume: 47 ISSN: 0014-4800 ISO Abbreviation: Exp. Mol. Pathol. Publication Date: 1987 Aug |
Date Detail:
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Created Date: 1987-09-08 Completed Date: 1987-09-08 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370711 Medline TA: Exp Mol Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 90-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Thoracic / physiopathology, ultrastructure* Arteriosclerosis / pathology, physiopathology* Body Weight Cholesterol / blood Cholesterol, Dietary / administration & dosage Dietary Fats / administration & dosage Fluorescence Polarization Hypercholesterolemia / pathology, physiopathology* Male Membrane Fluidity* Microsomes / physiology, ultrastructure* Muscle, Smooth, Vascular / ultrastructure Swine Vasomotor System / physiopathology |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol, Dietary; 0/Dietary Fats; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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