Document Detail


Effects of hydroxylamine, digitonin and triton X-100 on photoreceptor (paraflagellar swelling) and photoreception of Euglena gracilis.
MedLine Citation:
PMID:  8266645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present experiments that test the effects of agents commonly used in visual pigment investigations, namely hydroxylamine (NH2OH), digitonin and triton X-100, on the photoreceptor and photoreception of Euglena. Hydroxylamine reacts with free and opsin-bound retinal, in aqueous solution, to form stable oximes, whereas digitonin and triton X-100 are the most common extractants of rhodopsin. Since previous data indicate that the chromophore present in Euglena photoreceptor is retinal, we investigated the influence of these chemicals on this organelle. The effects of these agents were studied by means of phase contrast, fluorescence and transmission electron microscopy and photobehaviour experiments. Hydroxylamine inhibited the formation of the Euglena photoreceptor. Photoaccumulation experiments on hydroxylamine-treated cells showed that they are unable to perceive light. Digitonin solubilized the crystalline structure of the photoreceptor, whereas the triton effect was limited to the membranous structures of the cell, leaving the photoreceptor unimpaired.
Authors:
L Barsanti; V Passarelli; P Lenzi; P L Walne; J R Dunlap; P Gualtieri
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Vision research     Volume:  33     ISSN:  0042-6989     ISO Abbreviation:  Vision Res.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1994-01-25     Completed Date:  1994-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0417402     Medline TA:  Vision Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2043-50     Citation Subset:  IM    
Affiliation:
CNR Istituto di Biofisica, Pisa, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Digitonin / pharmacology*
Euglena gracilis / physiology*
Hydroxylamine
Hydroxylamines / pharmacology*
Microscopy, Electron, Scanning Transmission
Microscopy, Phase-Contrast
Octoxynol / pharmacology*
Photoreceptor Cells, Invertebrate / drug effects*,  physiology,  ultrastructure
Chemical
Reg. No./Substance:
0/Hydroxylamines; 11024-24-1/Digitonin; 7803-49-8/Hydroxylamine; 9002-93-1/Octoxynol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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