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Effects of hydrogen sulphide on motility patterns in the rat colon.
MedLine Citation:
PMID:  23297830     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: Hydrogen sulphide (H(2) S) is an endogenous gaseous signalling molecule with putative functions in gastrointestinal motility regulation. Characterization of H(2) S effects on colonic motility is crucial to establish its potential use as therapeutic agent in the treatment of colonic disorders. EXPERIMENTAL APPROACH: H(2) S effects on colonic motility were characterized using video recordings and construction of spatio-temporal maps. Microelectrode and muscle bath studies were performed to investigate the mechanisms underlying H(2) S effects. Sodium hydrosulphide (NaHS) was used as H(2) S source. KEY RESULTS: Rhythmic propulsive motor complexes (RPMCs) and ripples were observed in colonic spatio-temporal maps. Serosal addition of NaHS concentration-dependently inhibited RPMCs. In contrast, NaHS increased amplitude of the ripples without changing their frequency. Therefore, ripples became the predominant motor pattern. Neural blockade with lidocaine inhibited RPMCs, which were restored after administration of carbachol. Subsequent addition of NaHS caused RPMCs inhibition. Luminal addition of NaHS did not modify motility patterns. NaHS inhibited cholinergic excitatory junction potentials, carbachol-induced contractions and hyperpolarized smooth muscle cells but did not modify slow wave activity. CONCLUSIONS AND IMPLICATIONS: H(2) S is able to modulate colonic motility inhibiting propulsive contractile activity and enhancing the amplitude of ripples, promoting mixing. Muscle hyperpolarization and inhibition of neurally-mediated cholinergic responses contribute to the inhibitory effect on propulsive activity. H(2) S effects are not related to changes in the frequency of slow wave activity originating in the ICC network located near the submuscular plexus. Luminal H(2) S does not modify colonic motility probably because of epithelial detoxification.
Authors:
V Gil; S P Parsons; D Gallego; J D Huizinga; M Jimenez
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  British journal of pharmacology     Volume:  -     ISSN:  1476-5381     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. British Journal of Pharmacology © 2013 The British Pharmacological Society.
Affiliation:
Department of Cell Biology, Physiology and Immunology / Neuroscience Institute., Universitat Autònoma de Barcelona (UAB), Barcelona, Spain; Department of Medicine, Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada.
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