Document Detail


Effects of human recombinant interferon-alpha 2b on P450-dependent isozymes in rat liver and skin.
MedLine Citation:
PMID:  7632438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adult female Wistar rats divided into two groups (6 animals each) were subcutaneously treated with 10(5) units/day recombinant human interferon-alpha 2b (hrIFN-alpha 2b) and corresponding quantities of the vehicle, respectively, over a period of 7 days. Microsomal protein, P450 content, and the activities of aminopyrine-N-demethylase (ADM), 7-ethoxyresofurin-O-deethylase (7-ERO-D) and of erythromycin-N-demethylase (EMDM) were determined in the liver microsomes. Moreover, 7-ERO-D and EMDM activities were determined in the skin microsomes of the treated animals. In the liver microsomes hrIFN-alpha 2b caused a statistically significant increase in ADM activity and a statistically significant decrease in 7-ERO-D and EMDM activities, as compared to the controls. However, in the pooled skin microsomes 7-ERO-D activity showed a trend to increase under the administration of hrIFN-alpha 2b, whereas EMDM activity could not be detected in the treated or the control animals. The results of the present study indicate that hrIFN-alpha 2b is capable of affecting the activities of P450-dependent isozymes in the rat liver and skin in a different manner. Our findings support the hypothesis that clinically relevant interactions may occur during the concomitant administration of hrIFN-alpha 2b and other compounds that are metabolized by hepatic and cutaneous P450 monooxygenases.
Authors:
G Goerz; D Tsambaos; H C Schuppe; K Bolsen; S Georgiou; S Reinauer; C Zografakis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Skin pharmacology : the official journal of the Skin Pharmacology Society     Volume:  8     ISSN:  1011-0283     ISO Abbreviation:  Skin Pharmacol.     Publication Date:  1995  
Date Detail:
Created Date:  1995-09-11     Completed Date:  1995-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8810069     Medline TA:  Skin Pharmacol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  162-6     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Heinrich Heine University of Düsseldorf, Germany.
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MeSH Terms
Descriptor/Qualifier:
Aminopyrine N-Demethylase / metabolism
Animals
Aryl Hydrocarbon Hydroxylases*
Cytochrome P-450 CYP1A1
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme System / metabolism*
Female
Humans
Interferon Alfa-2b / pharmacology*
Isoenzymes / metabolism*
Liver / drug effects,  enzymology*
Microsomes / drug effects,  enzymology
Microsomes, Liver / drug effects,  enzymology
Oxidoreductases / metabolism
Oxidoreductases, N-Demethylating / metabolism
Rats
Rats, Wistar
Skin / drug effects,  enzymology*
Chemical
Reg. No./Substance:
0/Isoenzymes; 9035-51-2/Cytochrome P-450 Enzyme System; 99210-65-8/Interferon Alfa-2b; EC 1.-/Oxidoreductases; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/Cytochrome P-450 CYP1A1; EC 1.14.14.1/Cytochrome P-450 CYP3A; EC 1.5.-/Oxidoreductases, N-Demethylating; EC 1.5.3.-/Aminopyrine N-Demethylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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