Document Detail

Effects of a high-pressure treatment on the activity and structure of rabbit muscle proteasome.
MedLine Citation:
PMID:  16041125     Owner:  NLM     Status:  MEDLINE    
The effects were assessed of high hydrostatic pressure on the activity and structure of rabbit skeletal muscle proteasome. The pressure effects on the activity were measured by the amount of fluorometric products released from synthetic substrates under pressure and from fluorescein isothiocyanate (FITC)-labeled casein after releasing the pressure. The effects on the structure were measured by fluorescene spectroscopy under pressure, and by circular dichroism (CD) spectroscopy and surface hydrophobicity after releasing the pressure. The optimal pressure for the hydrolyzing activity of synthetic peptides was 50 MPa. The degradation of FITC-labeled casein increased linearly with increasing pressure applied up to 200 MPa, and then markedly decreased up to at 400 MPa. The changes in the tertiary structure detected by fluorometric measurement were irreversible, whereas the changes in the secondary structure were small compared with those by heat treatment. The pressure-induced activation of proteasome therefore seems to have been due to a little unfolding of the active sites of proteasome.
Shuhei Yamamoto; Yuichi Otsuka; Gerelt Borjigin; Kanna Masuda; Yoshihide Ikeuchi; Tadayuki Nishiumi; Atsushi Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  69     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-25     Completed Date:  2005-12-19     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1239-47     Citation Subset:  IM    
Graduate School of Science and Technology, Niigata University, Ikarashi, Niigata 950-2181, Japan.
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MeSH Terms
Caseins / chemistry,  metabolism
Circular Dichroism
Fluorescein-5-isothiocyanate / chemistry
Hydrostatic Pressure
Muscle, Skeletal / enzymology*
Oligopeptides / metabolism
Proteasome Endopeptidase Complex / chemistry,  isolation & purification,  metabolism*
Protein Structure, Secondary
Spectrometry, Fluorescence
Substrate Specificity
Reg. No./Substance:
0/Caseins; 0/Oligopeptides; 3326-32-7/Fluorescein-5-isothiocyanate; EC Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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