Document Detail


Effects of high pressure on glucose transport in the human erythrocyte.
MedLine Citation:
PMID:  12148839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of raised hydraulic pressure on D-glucose exit from human red cells at 25 degrees C were determined using light scattering measurements in a sealed pressurized spectrofluorimeter cuvette. The reduction in the rates of glucose exit with raised pressure provides an index of the activation volume, deltaV++ (delta ln k/deltaP)(T) = -deltaV++/RT. Raised pressure decreased the rate constant of glucose exit from 0.077 +/- 0.003 s(-1) to 0.050 +/- 0.002 s(-1) (n = 5, P < 0.003). The Ki for glucose binding to the external site was 2.7 +/- 0.4 mm (0.1 MPa) and was reduced to 1.45 +/- 0.15 mm (40 MPa), (P < 0.01, Student's t test). Maltose had a biphasic effect on deltaV++. At [maltose] <250 microM, deltaV++ of glucose exit increased above that with [maltose = 0 mM], at >1 mm maltose, deltaV++ was reduced below that with [maltose = 0 mM]. Pentobarbital (2 mM) decreased the deltaV++ of net glucose exit into glucose-free solution from 30 +/- 5 ml mol(-1) (control) to 2 +/- 0.5 ml mol(-1) (P < 0.01). Raised pressure had a negligible effect on L-sorbose exit. These findings suggest that stable hydrated and liganded forms of GLUT with lower affinity towards glucose permit higher glucose mobilities across the transporter and are modelled equally well with one-alternating or a two-fixed-site kinetic models.
Authors:
R J Naftalin; I Afzal; J A Browning; R J Wilkins; J C Ellory
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of membrane biology     Volume:  186     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-07-31     Completed Date:  2002-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  113-29     Citation Subset:  IM    
Affiliation:
Research Center for Cardiovascular Biology and Medicine, GKT School of Biomedical Sciences, King's College London, United Kingdom. richard.naftalin@kcl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Blood Glucose / metabolism*
Computer Simulation*
Erythrocytes / drug effects,  metabolism*
Glucose Transporter Type 1
Humans
Models, Biological*
Models, Chemical
Monosaccharide Transport Proteins / metabolism*
Pentobarbital / administration & dosage
Pressure
Sensitivity and Specificity
Sorbose / metabolism
Water / metabolism*
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Glucose Transporter Type 1; 0/Monosaccharide Transport Proteins; 0/SLC2A1 protein, human; 3615-39-2/Sorbose; 76-74-4/Pentobarbital; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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