Document Detail

Effects of high pressure on glucose transport in the human erythrocyte.
MedLine Citation:
PMID:  12148839     Owner:  NLM     Status:  MEDLINE    
The effects of raised hydraulic pressure on D-glucose exit from human red cells at 25 degrees C were determined using light scattering measurements in a sealed pressurized spectrofluorimeter cuvette. The reduction in the rates of glucose exit with raised pressure provides an index of the activation volume, deltaV++ (delta ln k/deltaP)(T) = -deltaV++/RT. Raised pressure decreased the rate constant of glucose exit from 0.077 +/- 0.003 s(-1) to 0.050 +/- 0.002 s(-1) (n = 5, P < 0.003). The Ki for glucose binding to the external site was 2.7 +/- 0.4 mm (0.1 MPa) and was reduced to 1.45 +/- 0.15 mm (40 MPa), (P < 0.01, Student's t test). Maltose had a biphasic effect on deltaV++. At [maltose] <250 microM, deltaV++ of glucose exit increased above that with [maltose = 0 mM], at >1 mm maltose, deltaV++ was reduced below that with [maltose = 0 mM]. Pentobarbital (2 mM) decreased the deltaV++ of net glucose exit into glucose-free solution from 30 +/- 5 ml mol(-1) (control) to 2 +/- 0.5 ml mol(-1) (P < 0.01). Raised pressure had a negligible effect on L-sorbose exit. These findings suggest that stable hydrated and liganded forms of GLUT with lower affinity towards glucose permit higher glucose mobilities across the transporter and are modelled equally well with one-alternating or a two-fixed-site kinetic models.
R J Naftalin; I Afzal; J A Browning; R J Wilkins; J C Ellory
Related Documents :
20374829 - Combined effect of enterocin as-48 and high hydrostatic pressure to control food-borne ...
17880189 - Effect of pressure and solvent on raman spectra of all-trans-beta-carotene.
11017609 - In situ x-ray study of thermal expansion and phase transition of iron at multimegabar p...
11027279 - Tryptophan permease gene tat2 confers high-pressure growth in saccharomyces cerevisiae.
17793559 - High-pressure transitions of germanium and a new high-pressure form of germanium.
21848329 - Gravimetric analysis of co2 adsorption on activated carbon at various pressures and tem...
3391509 - Renal sodium retention in cirrhosis: tubular site and relation to hepatic dysfunction.
10573769 - Natural pressure-volume curves and conventional cystometry.
19163979 - In vivo non-invasive high resolution mr-based method for the determination of the elast...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of membrane biology     Volume:  186     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-07-31     Completed Date:  2002-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  113-29     Citation Subset:  IM    
Research Center for Cardiovascular Biology and Medicine, GKT School of Biomedical Sciences, King's College London, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Transport
Blood Glucose / metabolism*
Computer Simulation*
Erythrocytes / drug effects,  metabolism*
Glucose Transporter Type 1
Models, Biological*
Models, Chemical
Monosaccharide Transport Proteins / metabolism*
Pentobarbital / administration & dosage
Sensitivity and Specificity
Sorbose / metabolism
Water / metabolism*
Reg. No./Substance:
0/Blood Glucose; 0/Glucose Transporter Type 1; 0/Monosaccharide Transport Proteins; 0/SLC2A1 protein, human; 3615-39-2/Sorbose; 76-74-4/Pentobarbital; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Are evidenced-based practice and best practice the same?
Next Document:  Purinergic-induced ion current in monolayers of C7-MDCK cells: role of basolateral and apical ion tr...