Document Detail


Effects of high-intensity intermittent swimming on PGC-1alpha protein expression in rat skeletal muscle.
MedLine Citation:
PMID:  15847644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The aim of the present investigation was to elucidate the effects of exercise intensity on exercise-induced expression of peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) protein in rat skeletal muscle. METHODS: We measured PGC-1alpha content in the skeletal muscles of male Sprague-Dawley rats (age: 5-6 weeks old; body weight: 150-170 g) after a single session of high-intensity intermittent exercise (HIE) or low-intensity prolonged swimming exercise (LIE). During HIE, the rats swam for fourteen 20-s periods carrying a weight (14% of body weight), and the periods of swimming were separated by a 10-s pause. LIE rats swam with no load for 6 h in two 3-h sessions, separated by 45 min of rest. RESULTS: After HIE, the PGC-1alpha protein content in rat epitrochlearis muscle had increased by 126, 140 and 126% at 2, 6 and 18 h, respectively, compared with that of the age-matched sedentary control rats' muscle. Immediately, 6 and 18-h after LIE, the PGC-1alpha protein content in the muscle was significantly elevated by 84, 95 and 67% respectively. The PGC-1alpha protein content observed 6 h after HIE tended to be higher than that observed after LIE. However, there was no statistically significant difference between the two values (P = 0.12). CONCLUSION: The present investigation suggests that irrespective of the intensity of the exercise, PGC-1alpha protein content in rat skeletal muscle increases to a comparable level when stimuli induced by different protocols are saturated. Further, HIE is a potent stimulus for enhancing the expression of PGC-1alpha protein, which may induce mitochondrial biogenesis in exercise-activated skeletal muscle.
Authors:
S Terada; K Kawanaka; M Goto; T Shimokawa; I Tabata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  184     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-25     Completed Date:  2005-09-22     Revised Date:  2009-08-12    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  England    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Affiliation:
Division of Health Promotion and Exercise, Incorporated Administrative Agency, National Institute of Health and Nutrition, Toyama, Shinjuku City, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Kinase / metabolism
Animals
Glycogen / analysis
Male
Muscle Proteins / metabolism
Muscle, Skeletal / enzymology,  metabolism*
RNA-Binding Proteins / analysis*
Rats
Rats, Sprague-Dawley
Swimming / physiology*
Transcription Factors / analysis*
Chemical
Reg. No./Substance:
0/Muscle Proteins; 0/Ppargc1a protein, rat; 0/RNA-Binding Proteins; 0/Transcription Factors; 9005-79-2/Glycogen; EC 2.7.4.3/Adenylate Kinase

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