Document Detail


Effects of high hydrostatic pressure treatments on haemagglutination activity and structural conformations of phytohemagglutinin from red kidney bean (Phaseolus vulgaris).
MedLine Citation:
PMID:  23194535     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Red kidney beans were subjected to high hydrostatic pressure (HHP) treatment (50, 150, 250, 350, 450MPa) and phytohaemagglutinin (PHA) was then extracted by affinity chromatography. It appeared that HHP treatment could increase crude extract yield and decrease its haemagglutination activity. For purified samples, PHA yield was not affected at pressures <450MPa while the haemagglutination activity was noticeably reduced at 450MPa. The structural changes were investigated using electrophoresis, size exclusion chromatography (SEC), Fourier transform infrared (FTIR) and differencial scanning calorimetry (DSC). Electrophoresis and SEC profiles revealed a new high molecular weight polymer after 450MPa treatment. At pressures <450MPa, FTIR showed an increase in β-sheet structure and a decrease in α-helix. At 450MPa, the bands at 1688cm(-1), representing aggregate strands and random coils, increased. The conclusions are that pressures <450MPa can cause PHA unfolding and induce PHA aggregation at 450MPa.
Authors:
Cencen Liu; Mouming Zhao; Weizheng Sun; Jiaoyan Ren
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Publication Detail:
Type:  Journal Article     Date:  2012-09-29
Journal Detail:
Title:  Food chemistry     Volume:  136     ISSN:  0308-8146     ISO Abbreviation:  Food Chem     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702639     Medline TA:  Food Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1358-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China.
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