Document Detail


Effects of high hydrostatic pressure on bacterial growth on human ossicles explanted from cholesteatoma patients.
MedLine Citation:
PMID:  22291908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: High hydrostatic pressure (HHP) treatment can eliminate cholesteatoma cells from explanted human ossicles prior to re-insertion. We analyzed the effects of HHP treatment on the microbial flora on ossicles and on the planktonic and biofilm states of selected isolates.
METHODOLOGY: Twenty-six ossicles were explanted from cholesteatoma patients. Five ossicles were directly analyzed for microbial growth without further treatment. Fifteen ossicles were cut into two pieces. One piece was exposed to HHP of 350 MPa for 10 minutes. Both the treated and untreated (control) pieces were then assessed semi-quantitatively. Three ossicles were cut into two pieces and exposed to identical pressure conditions with or without the addition of one of two different combinations of antibiotics to the medium. Differential effects of 10-minute in vitro exposure of planktonic and biofilm bacteria to pressures of 100 MPa, 250 MPa, 400 MPa and 540 MPa in isotonic and hypotonic media were analyzed using two patient isolates of Staphylococcus epidermidis and Neisseria subflava. Bacterial cell inactivation and biofilm destruction were assessed by colony counting and electron microscopy.
PRINCIPAL FINDINGS: A variety of microorganisms were isolated from the ossicles. Irrespective of the medium, HHP treatment at 350 MPa for 10 minutes led to satisfying but incomplete inactivation especially of gram-negative bacteria. The addition of antibiotics increased the efficacy of elimination. A comparison of HHP treatment of planktonic and biofilm cells showed that the effects of HPP were reduced by about one decadic logarithmic unit when HPP was applied to biofilms. High hydrostatic pressure conditions that are suitable to inactivate cholesteatoma cells fail to completely sterilize ossicles even if antibiotics are added. As a result of the reduced microbial load and the viability loss of surviving bacteria, however, there is a lower risk of re-infection after re-insertion.
Authors:
Steffen Dommerich; Hagen Frickmann; Jürgen Ostwald; Tobias Lindner; Andreas Erich Zautner; Kathleen Arndt; Hans Wilhelm Pau; Andreas Podbielski
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Publication Detail:
Type:  Journal Article     Date:  2012-01-23
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-31     Completed Date:  2012-06-04     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e30150     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, University of Rostock Hospital, Rostock, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Bacteria / growth & development*
Biofilms / growth & development
Cholesteatoma, Middle Ear / microbiology*,  pathology,  surgery
Colony Count, Microbial
Ear Ossicles / microbiology*,  pathology
Humans
Hydrostatic Pressure
Microbial Sensitivity Tests
Microbial Viability
Middle Aged
Neisseria / growth & development,  physiology
Otitis Media, Suppurative / microbiology
Staphylococcus epidermidis / growth & development,  physiology
Young Adult
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