Document Detail

Effects of high glucose plus high insulin on proliferation and apoptosis of mouse endothelial progenitor cells.
MedLine Citation:
PMID:  19109737     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To study the effects of high glucose plus high insulin (GI) on proliferation, apoptosis, morphology and differentiation of endothelial progenitor cells (EPC). METHODS: EPC were isolated and identified by magnetic cell sorting and flow cytometry. EPC proliferation and apoptosis were measured by MTT assay and Annexin-V/PI double labeling, respectively. Cell proliferation- and apoptosis-related factors were examined by Western blotting. RESULTS: Seven days after GI treatment, EPC were arrested at the G(0)/G(1) phase. Treatment with high glucose alone (HG) or GI but not HI (high insulin alone) decreased EPC proliferation and their expression of cyclin E, cdk2 and PCNA compared to control. The inhibitory effects of HG on EPC proliferation and growth-related proteins were more significant than those of GI. HG, GI and HI promoted EPC apoptosis along with caspase-3 overexpression. CONCLUSION: The result indicated that GI-induced apoptosis and growth inhibition might be one of mechanisms of EPC reduction and dysfunction in diabetes.
W Zhang; X Wang; H Jin; R Qian; G Zhang; S Chen; R Hu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  57     ISSN:  1420-908X     ISO Abbreviation:  Inflamm. Res.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-25     Completed Date:  2009-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  571-6     Citation Subset:  IM    
Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
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MeSH Terms
Apoptosis / drug effects*
Bone Marrow Cells / cytology,  physiology
Caspase 3 / metabolism
Cell Cycle / physiology
Cell Proliferation / drug effects*
Endothelial Cells / cytology,  drug effects*,  physiology*
Glucose / pharmacology*
Immunomagnetic Separation
Insulin / pharmacology*
Mice, Inbred BALB C
Stem Cells / cytology,  drug effects*,  physiology*
Reg. No./Substance:
11061-68-0/Insulin; 50-99-7/Glucose; EC 3.4.22.-/Caspase 3

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